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| Status |
Public on Jul 20, 2021 |
| Title |
Mesomelic Dysplasias Associated With The HOXD Locus Are Caused By Regulatory Reallocations [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Some human families display severe shortening and bending of the radius and ulna, a condition referred to as mesomelic dysplasia. Many of these families contain chromosomal rearrangements at 2q31, where the human HOXD locus maps. In mice, the dominant X-ray-induced Ulnaless inversion of the HoxD gene cluster produces a similar phenotype suggesting that the same pathological mechanism is at work in humans and mice. A tentative hypothesis was proposed where the various alterations to the genomic structure of HOXD could translocate Hoxd13 near to proximal limb enhancers, leading to its deleterious gain-of-expression in the embryonic forelimb. We evaluated this hypothesis by engineering a ca. 1Mb large inversion including the HoxD gene cluster, in order to position Hoxd13 within a chromatin domain rich in proximal limb enhancers. We show that these enhancers contact and activate Hoxd13 in proximal cells, concomitant to the formation of a mesomelic dysplasia phenotype. A secondary mutation in the coding frame of the HOXD13 protein in-cis with the inversion completely rescued the limb alterations, demonstrating that ectopic HOXD13 is indeed the unique cause of this bone anomaly. Single cell expression analysis and evaluation of HOXD13 binding sites in cells from this ectopic expression domain suggests that the phenotype arises primarily by acting through genes normally controlled by HOXD13 in distal limb cells. Altogether, these results provide a conceptual and mechanistic framework to understand and unify the molecular origins of human mesomelic dysplasia associated with 2q31.
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| Overall design |
Embryos were collected and stored in 1X PBS treated with DEPC and held on ice while genotyping was performed. Embryos with the desired genotype were selected and the posterior portion of each of the forelimbs was isolated for each replicate. The cells were digested in collagenase and stored in 1X PBS containing 10% FCS and 0.2mM EDTA to prevent cellular aggregation. GEM formation was performed with 10X protocol according to the 3.1 chemistry protocol.
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| Contributor(s) |
Bolt CC, Mascrez B, Lopez-Delisle L, Duboule D |
| Citation(s) |
34408147 |
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| Submission date |
Jan 25, 2021 |
| Last update date |
Nov 09, 2021 |
| Contact name |
Lucille Lopez-Delisle |
| E-mail(s) |
lucille.delisle@epfl.ch
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| Organization name |
EPFL
|
| Street address |
Station 19
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (3) |
| GSM5034677 |
E12.5 wild type posterior proximal forelimb scRNA-seq |
| GSM5034678 |
E12.5 inv2 posterior proximal forelimb, replicate 1 scRNA-seq |
| GSM5034679 |
E12.5 inv2 posterior proximal forelimb, replicate 2 scRNA-seq |
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| This SubSeries is part of SuperSeries: |
| GSE165495 |
Mesomelic Dysplasias Associated With The HOXD Locus Are Caused By Regulatory Reallocations |
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| Relations |
| BioProject |
PRJNA694687 |
| SRA |
SRP303203 |
