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| Status |
Public on Jun 10, 2010 |
| Title |
Expression data from lenses triply deficient for E2F1, E2F2, and E2F3 transcription factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The E2F family consists of transcriptional repressors and activators that control cell proliferation. In the classic paradigm of cell cycle regulation, the three activators, E2F1, E2F2 and E2F3, are invariably depicted as the final components of a CDK/Rb signaling cascade that executes the transcriptional program necessary to commit cells to enter S phase.
Unexpectedly, we find through analysis of Affymetrix expression array data that mature lens epithelial cells deficient for E2F1-3 fail to repress cell cycle-regulated genes (and other targets of E2F) and that this corresponds with subsequent apoptosis and cellular collapse in the lens.
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| Overall design |
Murine lenses were collected at two stages of development for RNA extraction and hybridization on Affymetrix microarrays. Our aim was to determine key events that lead to cellular collapse of lenses triply deficient for E2F1, E2F2, and E2F3 in neonates.
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| Contributor(s) |
Wenzel PL, Leone G |
| Citation(s) |
21185283 |
| |
| Submission date |
Jun 10, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Pamela L. Wenzel |
| E-mail(s) |
pamela.wenzel@childrens.harvard.edu
|
| Phone |
617-919-2016
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| Fax |
617-730-0222
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| Organization name |
Children's Hospital Boston
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| Department |
Hematology/Oncology
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| Lab |
George Daley
|
| Street address |
300 Longwood Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA116103 |
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