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| Status |
Public on Jul 20, 2021 |
| Title |
Real-time quantitative PCR analysis of PMA differentiated THP-1 infected with H37Rv or M. marinum, or heat killed H37Ra (HKMT) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
THP-1 cells were differentiated into macrophages with 0,1 µM PMA overnight then infected for 2 days with either H37Rv, M. marinum or heat killed H37Ra. Then RNA was collected in order to quantify the inflammatory response transcription as compared with the relative uninfected controls.
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| Overall design |
RNA quantity was normalized by using 500 ng Total RNA of each sample pror to RT-PCR. Each sample was treated equally.
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| |
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| Contributor(s) |
Bade P, Simonetti F, Sans S, Laboudie P, Kissane K, Chappat N, Lagrange S, Apparailly F, Roubert C, Duroux-Richard I |
| Citation(s) |
34276658 |
| |
| Submission date |
Jan 22, 2021 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Pauline Bade |
| E-mail(s) |
bade_pauline@hotmail.com
|
| Organization name |
Evotec LYON ID SAS
|
| Street address |
40 avenue Tony Garnier
|
| City |
Lyon |
| ZIP/Postal code |
69160 |
| Country |
France |
| |
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| Platforms (1) |
| GPL29639 |
Custom TaqMan® Gene Expression Array Cards Inflammatory Response [Catalog# 4342253] |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA694111 |
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