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| Status |
Public on Feb 10, 2021 |
| Title |
Single-cell RNA sequencing of healthy and inflamed murine neutrophils |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Dataset containing single-cell RNA sequencing data of >17,000 healthy and perturbed murine neutrophils across biological tissues. This study contains expression data from healthy tissues (bone marrow, spleen, peripheral blood) as well as models of experimental (K/BxN serum transfer arthritis: peripheral blood and joint, IL-1β peritonitis and IL-1β pneumonitis). Single cell RNA-sequencing was performed on the 10X platform
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| |
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| Overall design |
CD11b+ Ly6G+ PI-negative live neutrophils were sorted from healthy tissues (bone marrow, spleen, peripheral blood) and models of experimental inflammation (K/BxN serum transfer arthritis: peripheral blood and joint, IL-1β peritonitis and IL-1β pneumonitis) and single cell RNA-sequencing was performed on the 10X platform.
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| |
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| Contributor(s) |
Grieshaber-Bouyer R, Nigrovic P |
| Citation(s) |
34001893 |
| |
| Submission date |
Jan 21, 2021 |
| Last update date |
Jun 08, 2021 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA693858 |
| SRA |
SRP302737 |
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