GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE165263 Query DataSets for GSE165263
Status Public on Nov 29, 2021
Title miR profiling of apoptotic murine neurons and their supernatent
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary miRNAs were recently discovered to directly activate receptors thereby extending their original role beyond post-transcriptional gene expression regulation. In particular, Here, conducting a systematic screening approach we identified miRNAs that in extracellular form can serve as receptor ligands, and elaborated the consequences of such an interaction in CNS injury.
Primary cortical neurons isolated from C57BL/6 mice were first exposed to staurosporine, a potent, non-selective kinase inhibitor, which is widely used to model neuronal apoptosis, for 8 h. While neurons remained morphologically intact within this time period, efficient apoptosis induction through activation of neuronal caspase-3 signaling was verified by western blot. Next, neurons and corresponding supernatant (S/N) were separately harvested and processed for miRNA detection. After enrichment and isolation of the small RNA fractions from neurons and their corresponding S/N, they were analyzed by GeneChip miRNA 4.0 array technology. Microarray data were processed using the robust multi-array average (RMA) method on the R/Bioconductor platform. Most of subsequent analysis was restricted to miRNA probes that were specific for Mus musculus.
Overall design Replicate samples were taken from neurons and their supernatant after induction of apoptosis. As control, samples were extracted from neurons and their supernatant without induced apoptosis. The abundance levels of miRNA were compared between apoptotic and control neurons as well as between the supernatents of apoptotic and of control neurons, respectively
Contributor(s) Futschik ME, Machado R, Wallach T, Lehnard S
Citation(s) 34838071
Submission date Jan 21, 2021
Last update date Dec 01, 2021
Contact name Matthias Futschik
Organization name Universidade do Algarve
Lab SysBioLab
Street address Campus de Gambelas
City Faro
ZIP/Postal code 8005-139
Country Portugal
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (20)
GSM5029178 Neurons, DMSO, replicate 1
GSM5029179 Neurons, Staurosporine, replicate 1
GSM5029180 Neurons, DMSO, replicate 2
BioProject PRJNA693839

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165263_RAW.tar 14.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap