|
| Status |
Public on May 01, 2021 |
| Title |
B cells and their progenitors reside in homeostatic meninges |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The central nervous system (CNS) is ensheathed by the meninges and cerebrospinal fluid. Recent findings suggest complex immunological functions of these CNS-associated border tissues. Unlike myeloid lineage cells, lymphocytes in border compartments have been largely disregarded in previous studies. Based on single cell transcriptomics, we here identified a highly location-specific composition and expression profile of tissue-resident leukocytes in every border compartment. Only the dura layer of the meninges contained a large population of B cells under homeostatic conditions in multiple species. Murine dura B cells exhibited slow turnover, long-term tissue residency and matured in experimental neuroinflammation. The dura also contained B lineage progenitors at the pro-B cell stage unexpected outside of bone marrow, without direct influx from the periphery or the skull bone marrow. This identifies the dura as an unexpected site of B cell residence and potentially development in homeostasis and neuroinflammation.
|
| |
|
| Overall design |
Single-cell RNA sequencing of CNS-associated border compartments in rats and mice (naive and EAE)
|
| |
|
| Contributor(s) |
Schafflick D, Wolbert J, Heming M, Lu I, zu Hörste GM |
| Citation(s) |
34253922 |
| |
| Submission date |
Jan 20, 2021 |
| Last update date |
Jul 14, 2021 |
| Contact name |
I-Na Lu |
| E-mail(s) |
luina0416@gmail.com
|
| Organization name |
University Hospital Muenster
|
| Department |
Department of Neurology with Institute of Translational Neurology
|
| Lab |
Neuroimmunology
|
| Street address |
Mendelstr. 7
|
| City |
Muenster |
| State/province |
NRW |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (10)
|
| GSM5027154 |
single-cell RNA seq data of the central nervous system from rat [CNS] |
| GSM5027155 |
single-cell RNA seq data of the choroid plexus from rat [CP] |
| GSM5027156 |
single-cell RNA seq data of the dura from rat [Dura] |
| GSM5027157 |
single-cell RNA seq data of the pia from rat [Pia] |
| GSM5027158 |
single-cell RNA seq data of blood from rat [PBMC] |
| GSM5027159 |
single-cell RNA seq data of the cerebrospinal fluid from rat [CSF] |
| GSM5027160 |
single-cell RNA seq data of the dura from naive mice [dura2] |
| GSM5027161 |
single-cell RNA seq data of the dura from EAE mice [dura_a_E] |
| GSM5027162 |
single-cell BCR seq data of the dura from naive mice [dura-BCR] |
| GSM5027163 |
single-cell BCR seq data of the dura from EAE mice [dura-a-E-BCR] |
|
| Relations |
| BioProject |
PRJNA693439 |
| SRA |
SRP302510 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE165153_RAW.tar |
110.0 Kb |
(http)(custom) |
TAR (of CSV) |
| GSE165153_mouse_annotation_cluster.csv.gz |
45.4 Kb |
(ftp)(http) |
CSV |
| GSE165153_mouse_annotation_condition.csv.gz |
35.9 Kb |
(ftp)(http) |
CSV |
| GSE165153_mouse_barcodes.tsv.gz |
42.6 Kb |
(ftp)(http) |
TSV |
| GSE165153_mouse_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE165153_mouse_matrix.mtx.gz |
64.8 Mb |
(ftp)(http) |
MTX |
| GSE165153_rat_annotation_cluster.csv.gz |
111.4 Kb |
(ftp)(http) |
CSV |
| GSE165153_rat_annotation_tissue.csv.gz |
107.9 Kb |
(ftp)(http) |
CSV |
| GSE165153_rat_barcodes.tsv.gz |
131.5 Kb |
(ftp)(http) |
TSV |
| GSE165153_rat_features.tsv.gz |
285.1 Kb |
(ftp)(http) |
TSV |
| GSE165153_rat_matrix.mtx.gz |
73.3 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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