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| Status |
Public on May 18, 2022 |
| Title |
Unravelling the chromatin landscape and enhancer logic mediating spatiotemporal patterning of early cardiovascular progenitors [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The mammalian heart arises from various populations of Mesp1-expressing cardiovascular progenitors (CPs) that are specified during the early stages of gastrulation. Mesp1 acts as a master regulator of CP specification and differentiation. However, how Mesp1 regulates the chromatin landscape of nascent mesodermal cells to define the temporal and spatial patterning of the distinct populations of CP remains unknown. Here, by combining ChIP-seq, RNA-seq and ATAC-seq during mouse pluripotent stem cell differentiation, we defined the temporal remodelling of the chromatin landscape mediated by Mesp1. We identified different enhancers that are temporally regulated to erase the pluripotent state and specify the pools of CPs that mediate heart development. We found that Mesp1 acts as a pioneer transcription factor (TF) and identified Zic TFs as essential cofactors that regulate Mesp1 pioneer activity at key mesodermal enhancers, thereby regulating the chromatin remodelling and gene expression associated with the specification of the different populations of CPs in vivo. Our study identifies the dynamics of the chromatin landscape and enhancer remodelling associated with temporal patterning of early mesodermal cells into the distinct populations of CPs that mediate heart development.
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| Overall design |
assay for transposase-accessible chromatin using sequencing (ATAC-seq) on differentiating mouse pluripotent stem cells where the expression of Mesp1 is doxycycline-inducible
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| |
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| Contributor(s) |
Lin X, Swedlund B, Calonne E, Fuks F, Blanpain C |
| Citation(s) |
35817961 |
| |
| Submission date |
Jan 19, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Cedric Blanpain |
| E-mail(s) |
cedric.blanpain@ulb.ac.be
|
| Organization name |
Université Libre de Bruxelles (ULB)
|
| Lab |
Laboratory of Stem Cells and Cancer
|
| Street address |
Route de Lennik 808, Bat C, C6-126
|
| City |
Brussels |
| State/province |
anderlecht |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platforms (3) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (25)
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| This SubSeries is part of SuperSeries: |
| GSE165107 |
Unravelling the chromatin landscape and enhancer logic mediating spatiotemporal patterning of early cardiovascular progenitors |
|
| Relations |
| BioProject |
PRJNA693269 |
| SRA |
SRP302364 |
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