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| Status |
Public on Jan 21, 2021 |
| Title |
ChIP-seq of Runx2-FLAG in primary chondrocytes. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: To demonstrate the role of Transcription factor Runx2 in primary chondrocytes with or without IL-1beta. Method: Fragmented DNA samples were collected from primary chondrocytes of 5-day-old Runx2-Biotin-FLAG-tag mice, cultured with or without IL-1beta. Results: More than 20,000 and 10,000 peaks were gained from chondrocytes without and with IL-1beta, resepectively. Conclusions: Runx2 are associated cellular process and extracellular matrics transcription in primary chondrocytes.
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| |
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| Overall design |
DNA fragment gathered by Anti-Flag antibody or Anti-IgG antibody from primary chondrocytes of 5-day old Runx2-Biotin-FLAG-tag mice.
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| Contributor(s) |
Nagata K, Saito T |
| Citation(s) |
36261443 |
| |
| Submission date |
Jan 19, 2021 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Kosei Nagata |
| E-mail(s) |
knagata-tky@umin.ac.jp
|
| Organization name |
the University of Tokyo
|
| Department |
Graduate School of Medicine
|
| Lab |
Sensory and Motor System Medicine
|
| Street address |
Hongo7-3-1
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA693553 |
| SRA |
SRP302586 |
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