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| Status |
Public on Jan 21, 2021 |
| Title |
RNA-seq of Runx2 cKO primary chondrocytes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To demonstrate Runx2's association with organizing the extracellular matrix in primary chondrocytes. Method: RNA samples were collected from primary chondrocytes of 5-day-old Col2a1-CreERT2;Runx2fl/fl and Runx2fl/fl mice, cultured with or without IL-1beta. Results: Thirty-three genes cultured without IL-1beta and 45 genes with IL-1beta were up- or down-regulated by more than 2-fold in the Runx2 knockout in primary chondrocytes. Conclusions: Genes including collagen fibers were down-regulated in Runx2 cKO primary chondrocytes.
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| |
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| Overall design |
Primary chondrocytes mRNA profiles of 5-day old Cntl and Runx2 cKO mice.
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| |
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| Contributor(s) |
Nagata K, Saito T |
| Citation(s) |
36261443 |
| |
| Submission date |
Jan 19, 2021 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Kosei Nagata |
| E-mail(s) |
knagata-tky@umin.ac.jp
|
| Organization name |
the University of Tokyo
|
| Department |
Graduate School of Medicine
|
| Lab |
Sensory and Motor System Medicine
|
| Street address |
Hongo7-3-1
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA693204 |
| SRA |
SRP302347 |
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