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| Status |
Public on Jun 10, 2009 |
| Title |
Arabidopsis thaliana gene expression changes upon treatment with green peach aphid saliva |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Myzus persicae (green peach aphid) feeding on Arabidopsis thaliana induces a defense response, quantified as reduced aphid progeny production, in infested leaves but not in other parts of the plant. Similarly, infiltration of aphid saliva into Arabidopsis leaves causes only a local increase in aphid resistance. Further characterization of the defense-eliciting salivary components indicates that Arabidopsis recognizes a proteinaceous elicitor with a size between 3 to 10 kD. Genetic analysis using well-characterized Arabidopsis mutant shows that saliva-induced resistance against M. persicae is independent of the known defense signaling pathways involving salicylic acid, jasmonate, and ethylene. Among 78 Arabidopsis genes that were induced by aphid saliva infiltration, 52 had been identified previously as aphid-induced, but few are responsive to the well-known plant defense signaling molecules salicylic acid and jasmonate. Quantitative PCR analysis confirms expression of saliva-induced genes. In particular, expression of a set of O-methyltransferases, which may be involved in the synthesis of aphid-repellent glucosinolates, was significantly up-regulated by both M. persicae feeding and treatment with aphid saliva. However, this did not correlate with increased production of 4-methoxyindol-3-ylmethylglucosinolate, suggesting that aphid salivary components trigger an Arabidopsis defense response that is independent of this aphid-deterrent glucosinolate.
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| Overall design |
3 biological replicates (control and treatment). Total number of samples: 6.
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| Contributor(s) |
Jander G, de Vos M |
| Citation(s) |
19558622 |
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| Submission date |
Jun 08, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Georg Jander |
| E-mail(s) |
gj32@cornell.edu
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| Phone |
+1 607 254 1365
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| URL |
http://www.bti.cornell.edu/GeorgJander.php
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| Organization name |
Boyce Thompson Institute for Plant Research
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| Street address |
1 Tower Rd
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA116115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16497_Jander_NormalizedFoldChange.txt.gz |
520.1 Kb |
(ftp)(http) |
TXT |
| GSE16497_RAW.tar |
13.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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