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| Status |
Public on Feb 15, 2022 |
| Title |
Single-cell transcriptional and epigenetic dynamics during aortic aging in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The impact of vascular aging (VA) has been extensively studied, yet little is known regarding the cellular and molecular mechanism underlying age-related VA in aortic cellular subpopulations. Herein, we analyzed transcriptomes and transposase-accessible chromatin profiles from aortas of 4-, 26-, and 86-week-old mice using single-cell RNA sequencing and ATAC sequencing. By integrating the heterogeneous transcriptome and chromatin accessibility data, we identified cell-specific transcription factor (TF) regulatory networks and open chromatin states. Moreover, we found that aortic aging affects cell interactions, inflammation, cell type composition and dysregulation of transcriptional control. Endothelial cell (EC) 1 has higher gene set activity related to senescence, aging, and adipogenesis than EC 2. Construction of senescence trajectories showed that the senescence of EC 1 and fibroblasts is associated with distinct TF open chromatin states and an mRNA expression model. Our data provide a system-wide model for transcriptional and epigenetic regulation during aortic aging at single-cell resolution.
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| Overall design |
Transcriptomes and transposase-accessible chromatin profiles from aortas of two 4-week-old, two 26-week-old, and two 86-week-old mice
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| Contributor(s) |
Xie W, Ke Y, You Q, Li J, Chen L, Li D, Fang J, Chen X, Zhou Y, Chen L, Hong H |
| Citation(s) |
34879708 |
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| Submission date |
Jan 11, 2021 |
| Last update date |
Feb 16, 2022 |
| Contact name |
Wenhui Xie |
| E-mail(s) |
doctorxiewenhui@fjmu.edu.cn
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| Phone |
8615695907499
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| Organization name |
Fujian Medical University Union Hospital
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| Department |
Department of Geriatrics, Fujian Key Laboratory of Vascular Aging (Fujian Medical University)
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| Street address |
Gulou District, Xinquan Road, No.29
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| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350001 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA691260 |
| SRA |
SRP301266 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164585_RAW.tar |
753.1 Mb |
(http)(custom) |
TAR (of H5, MTX, TSV) |
| GSE164585_merge_UMI_count.txt.gz |
69.5 Mb |
(ftp)(http) |
TXT |
| GSE164585_metadata.txt.gz |
177.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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