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| Status |
Public on Apr 01, 2021 |
| Title |
Defining the lineage of thermogenic perivascular adipose tissue [Adult Aorta] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Brown adipose tissue can expend large amounts of energy and thus increasing its amount or activity is a promising therapeutic approach to combat metabolic disease. In humans, major deposits of brown fat cells are found intimately associated with large blood vessels, corresponding to perivascular adipose tissue (PVAT). However, the cellular origins of PVAT are poorly understood. We applied single cell transcriptomic analyses, ex vivo adipogenesis assays, and genetic fate mapping to determine the identity of perivascular adipocyte progenitors. In mice, we found that thoracic PVAT develops from a fibroblastic lineage, consisting of progenitor cells (Pdgfra+; Ly6a+; Pparg-) and preadipocytes (Pdgfra+; Ly6a+; Pparg+). Progenitor and preadipocyte cells in PVAT shared transcriptional similarity with analogous cell types in white adipose tissue, pointing towards a conserved hierarchical structure of adipose lineage cells. Interestingly, the aortic adventitia of adult animals contained a novel population of adipogenic smooth muscle cells (SMCs) (Myh11+; Pdgfra-; Pparg+) that contributed to perivascular adipocyte formation. Similarly, human PVAT contained presumptive fibroblastic and SMC-like adipocyte progenitors, as revealed by single nucleus RNAseq. Taken together, these studies define distinct populations of progenitor cells for thermogenic PVAT, providing a foundation for developing strategies to augment brown fat activity
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| Overall design |
Transcriptome (mRNA) profiles of FACS isolated cell populations from the aorta and perivascular fat ( intermediate cells, progenitor cells, and smooth muscle cell (SMC) populations SMC1 and SMC2) from wild type (WT) adult (13 week old) male mice were generated by deep sequencing, in triplicate, followed by DESeq2 analysis for differential expression of genes between the various groups.
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| Contributor(s) |
Seale P, Angueira A, Sakers A |
| Citation(s) |
33846639 |
| |
| Submission date |
Jan 10, 2021 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Patrick Seale |
| E-mail(s) |
sealep@pennmedicine.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Street address |
Smilow Center for Translational Research, 12th Floor 3400 Civic Center Blvd.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| GSM5013327 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Intermediate Cells [Ao1-Int] |
| GSM5013328 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Progenitor Cells [Ao1-Prog] |
| GSM5013329 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC1 Cells [Ao1-SMC_1] |
| GSM5013330 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC2 Cells [Ao1-SMC_2] |
| GSM5013331 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Intermediate Cells [Ao2-Int] |
| GSM5013332 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Progenitor Cells [Ao2-Prog] |
| GSM5013333 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC1 Cells [Ao2-SMC_1] |
| GSM5013334 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC2 Cells [Ao2-SMC_2] |
| GSM5013335 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Intermediate Cells [Ao3-Int] |
| GSM5013336 |
Bulk RNA Seq of FACS Sorted Adult Perivascular Progenitor Cells [Ao3-Prog] |
| GSM5013337 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC1 Cells [Ao3-SMC_1] |
| GSM5013338 |
Bulk RNA Seq of FACS Sorted Adult Perivascular SMC2 Cells [Ao3-SMC_2] |
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| This SubSeries is part of SuperSeries: |
| GSE164528 |
Defining the lineage of thermogenic perivascular adipose tissue |
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| Relations |
| BioProject |
PRJNA691056 |
| SRA |
SRP301140 |
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