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Series GSE164336 Query DataSets for GSE164336
Status Public on Dec 21, 2022
Title In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During embryogenesis, haematopoietic and endothelial lineages emerge closely in time and space. It is thought that the first blood and endothelium derive from a common clonal ancestor, the haemangioblast. However, investigation of candidate haemangioblasts in vitro revealed the capacity for mesenchymal differentiation, a feature more compatible with an earlier mesodermal precursor. To date, no evidence for an in vivo haemangioblast has been discovered. Using single cell RNA-Sequencing and in vivo cellular barcoding, we have unraveled the ancestral relationships that give rise to the haematopoietic lineages of the yolk sac, the endothelium, and the mesenchyme. We show that the mesodermal derivatives of the yolk sac are produced by three distinct precursors with dual-lineage outcomes: the haemangioblast, the mesenchymoangioblast, and a previously undescribed cell type: the haematomesoblast. Between E5.5 and E7.5, this trio of precursors seeds haematopoietic, endothelial, and mesenchymal trajectories.
 
Overall design Yolk sac lineages related to emergenece of blood and endothelial cells were collected between E7.5 and E10.5 of mouse embryonic development using Flk1-GFP mouse (Shalaby et al, DOI: 10.1016/s0092-8674(00)80283-4) and combinations of antibodies described in (Potts et al, https://doi.org/10.1182/blood-2014-02-559468).
 
Contributor(s) Choi J, Potts KS, Biben C, Weber TS, Miles DC, Carmagnac A, Sargeant T, de Graaf CA, Fennell KA, Farley A, Stonehouse O, Dawson MA, Hilton DJ, Naik SH, Taoudi S
Citation(s) 36596806
Submission date Jan 06, 2021
Last update date Jan 13, 2023
Contact name Jarny Choi
E-mail(s) jarnyc@unimelb.edu.au
Organization name Centre for Stem Cell Systems
Department Anatomy and Neuroscience
Lab Wells Lab
Street address The University of Melbourne
City Melbourne
State/province Victoria
ZIP/Postal code 3010
Country Australia
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (1073)
GSM5006705 Primitive erythrocytes (EryP) E8.5 [E8.5 EryP_0]
GSM5006706 Endothelium (Endo) E8.5 [E8.5 Endo_0]
GSM5006707 Erythro-Myeloid Progenitors (EMP) E8.25 [E8.5 PreHPC_0]
Relations
BioProject PRJNA690061
SRA SRP300627

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE164336_counts.3.0.txt.gz 11.6 Mb (ftp)(http) TXT
GSE164336_counts.Unfiltered.3.0.txt.gz 14.5 Mb (ftp)(http) TXT
GSE164336_cpm.Size.3.0.txt.gz 68.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
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