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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 16, 2021 |
| Title |
Concurrently expressed tumor neoantigens drive phenotypically distinct CD8 T cell responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
CD8 T cell responses against different tumor neoantigens occur simultaneously, yet little is known about the interplay of these responses or its impact on tumor control. Here, we characterized the CD8 T cell response against two co-expressed tumor neoantigens, SIINFEKL (SIIN) and SIYRYYGL (SIY), in a genetically engineered mouse model of lung adenocarcinoma. In this model, tumors are initiated in KrasLSLG12D;p53fl/fl (KP) mice by intratracheal installation of lentiviruses expressing Cre recombinase and the antigens as a fusion to luciferase (LucOS). In these KP LucOS mice, the CD8 T cell response peaks around 5 weeks post-tumor initiation and is dominated by expansion of cells specific to SIIN. SIY drives a much smaller T cell response, but it persists better over time. To further investigate phenotypic differences in the T cell responses against SIIN versus SIY, we subjected antigen-specific CD8 T cells from 5 week mice to paired single cell RNA and TCR sequencing using the 10X Genomics platform.
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| Overall design |
SIIN- and SIY-specific CD8 T cells were sorted from the lungs of KP LucOS mice 5 weeks post-tumor initiation and subjected to paired RNA and TCR single cell RNA sequencing using the 10X Genomics platform.
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| Contributor(s) |
Burger ML, Cruz AM, Bhutkar A, Blatt S, Jacks T |
| Citation(s) |
34534464 |
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| Submission date |
Jan 04, 2021 |
| Last update date |
Dec 17, 2021 |
| Contact name |
Amanda Margarita Cruz |
| E-mail(s) |
amcruz@mit.edu
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| Organization name |
Massachusetts Institute of Technology
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| Department |
Biology
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| Lab |
Tyler Jacks
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| Street address |
500 Main Street, 76-431
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (2) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA689431 |
| SRA |
SRP300162 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164177_RAW.tar |
230.1 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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