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| Status |
Public on Jan 13, 2021 |
| Title |
Serine biosynthesis is a metabolic vulnerability in FLT3-ITD-driven acute myeloid leukaemia [iFLT3-ITD-Depletion_In VIVO] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed 3'-RNA-Sequencing on RNA isolated from murine MLL-AF9/iFLT3-ITD cells where the inducible iFLT3-ITD transgene had been depleted for 48 hours compared to non-depleted conditions in vivo to perform differential gene expression analysis.
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| Overall design |
1 million MLL-AF9/iFLT3-ITD cells were transplanted into NSG mice via tail vein IV injection. Mice were fed doxycycline-containing chow and water to maintain inducible iFLT3-ITD transgene expression, and leukaemias were allowed to engraft for 96 hours. Half of the cohort of mice (n=3) was then placed on regular chow and water to deplete inducible iFLT3-ITD transgene expression for 48 hours, while the other half of the cohort (n=3) remained on doxycycline-containing chow and water to maintain inducible iFLT3-ITD transgene expression as a control. Mice were all culled after 48 hours and leukaemic cells sorted from the bone marrow. Total RNA was isolated from cells and 3'-RNA libraries prepared for next-generation sequencing.
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| Contributor(s) |
Bjelosevic S, Gruber E, Brown KK, Johnstone RW |
| Citation(s) |
33436370 |
| |
| Submission date |
Dec 28, 2020 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Stefan Bjelosevic |
| E-mail(s) |
stefan.bjelosevic@petermac.org
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| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Translational Haematology Program
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| Street address |
305 Grattan Street
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| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3000 |
| Country |
Australia |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| GSM4990769 |
MLL-AF9/iFLT3-ITD In Vivo ON Replicate 1 |
| GSM4990770 |
MLL-AF9/iFLT3-ITD In Vivo ON Replicate 2 |
| GSM4990771 |
MLL-AF9/iFLT3-ITD In Vivo ON Replicate 3 |
| GSM4990772 |
MLL-AF9/iFLT3-ITD OFF In Vivo 48 hrs Replicate 1 |
| GSM4990773 |
MLL-AF9/iFLT3-ITD OFF In Vivo 48 hrs Replicate 2 |
| GSM4990774 |
MLL-AF9/iFLT3-ITD OFF In Vivo 48 hrs Replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE163932 |
Serine biosynthesis is a metabolic vulnerability in FLT3-ITD-driven acute myeloid leukaemia |
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| Relations |
| BioProject |
PRJNA688230 |
| SRA |
SRP299459 |
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