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| Status |
Public on Dec 29, 2020 |
| Title |
ZFP541 ChIP-seq targets during male meiotic prophase |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ZFP541 is assumed to possess putative DNA-binding domains and associated with histone deacetylases, implying it plays a role in regulation of transcription via modulation of chromatin status. Therefore, we used ChIP-seq to search for targets of ZFP541 in meiotic prophase of mouse spermatocytes.
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| Overall design |
ZFP541 ChIP was performed using chromatin lysates, that were derived from wild type testes at postnatal day 18-19, and protein A Dyna-beads (Thermo-Fisher) coupled with 5 μg of rabbit anti-ZFP541-N antibodies (#1 and #2). ChIP-seq libraries were prepared using 20 ng of input DNA, and 4 ng of ZFP541 ChIP DNA with KAPA Library Preparation Kit (KAPA Biosystems) and NimbleGen SeqCap Adaptor Kit A or B (Roche) and sequenced by Illumina Hiseq 1500 to obtain single end 50 nt reads. ChIP-seq reads were trimmed to remove adapter sequence when converting to a fastq file.
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| Contributor(s) |
Takada Y, Ishiguro K, Tachibana M, Maeda R, Matsuura K, Horisawa K |
| Citation missing |
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| Submission date |
Dec 28, 2020 |
| Last update date |
Dec 31, 2020 |
| Contact name |
Kei-ichiro Ishiguro |
| E-mail(s) |
ishiguro@kumamoto-u.ac.jp
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| Phone |
0963736607
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| Organization name |
Kumamoto university
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| Department |
Institute of Molecular Embryology and Genetics
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| Lab |
Department of Chromosome Biology
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| Street address |
2-2-1, Honjo
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| City |
Chuo-ku |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA688209 |
| SRA |
SRP299443 |
