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Series GSE163916 Query DataSets for GSE163916
Status Public on Dec 29, 2020
Title ZFP541 ChIP-seq targets during male meiotic prophase
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ZFP541 is assumed to possess putative DNA-binding domains and associated with histone deacetylases, implying it plays a role in regulation of transcription via modulation of chromatin status. Therefore, we used ChIP-seq to search for targets of ZFP541 in meiotic prophase of mouse spermatocytes.
Overall design ZFP541 ChIP was performed using chromatin lysates, that were derived from wild type testes at postnatal day 18-19, and protein A Dyna-beads (Thermo-Fisher) coupled with 5 μg of rabbit anti-ZFP541-N antibodies (#1 and #2). ChIP-seq libraries were prepared using 20 ng of input DNA, and 4 ng of ZFP541 ChIP DNA with KAPA Library Preparation Kit (KAPA Biosystems) and NimbleGen SeqCap Adaptor Kit A or B (Roche) and sequenced by Illumina Hiseq 1500 to obtain single end 50 nt reads. ChIP-seq reads were trimmed to remove adapter sequence when converting to a fastq file.
Contributor(s) Takada Y, Ishiguro K, Tachibana M, Maeda R, Matsuura K, Horisawa K
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Submission date Dec 28, 2020
Last update date Dec 31, 2020
Contact name Kei-ichiro Ishiguro
Phone 0963736607
Organization name Kumamoto university
Department Institute of Molecular Embryology and Genetics
Lab Department of Chromosome Biology
Street address 2-2-1, Honjo
City Chuo-ku
State/province Kumamoto
ZIP/Postal code 860-0811
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (3)
GSM4990661 input
GSM4990662 ZFP541 ChIP_Antibody1
GSM4990663 ZFP541 ChIP_Antibody2
BioProject PRJNA688209
SRA SRP299443

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Supplementary file Size Download File type/resource
GSE163916_RAW.tar 506.1 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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