|
| Status |
Public on Feb 22, 2022 |
| Title |
Transcriptional heterogeneity in primary and metastatic gastric cancer revealed using single-cell RNA sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Deciphering intra- and inter-tumoral heterogeneity is essential for understanding gastric cancer (GC) biology and identifying effective therapeutic targets. We used single-cell RNA sequencing (scRNA-seq) to reveal the transcriptional heterogeneity of diverse cells within primary and metastatic GC and their roles in disease progression.
|
| |
|
| Overall design |
We performed unbiased scRNA-seq analysis of 42,968 cells from 10 fresh human tissue samples from six patients. Primary tumor and adjacent non-tumoral samples and six metastases from different organs or tissues (liver, peritoneum, ovary, lymph node) were evaluated. Validation experiments were performed using histological assays and bulk transcriptomic datasets.
|
| |
|
| Contributor(s) |
Jiang H, Yu D, Yang P |
| Citation(s) |
35184420 |
| Submission date |
Dec 20, 2020 |
| Last update date |
Aug 03, 2022 |
| Contact name |
penghui Yang |
| E-mail(s) |
yangph@zju.edu.cn
|
| Phone |
18811381956
|
| Organization name |
Pharmaceutical Informatics Institute
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310007 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA689571 |
| SRA |
SRP300226 |
Less...
More...