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Series GSE163427 Query DataSets for GSE163427
Status Public on May 27, 2021
Title Totipotency of mouse zygotes extends to single blastomeres of 4-cell stage embryos
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In multicellular organisms, heterogametes of oocytes and sperms are fertilized and resulting zygotes give rise to new individuals. The ability of zygotes that produce a fully formed individual from single cell when placed in a supportive environment is defined as totipotency. Given that totipotent cells are the source of all multicellular organisms, better understanding of totipotency has a profound effect on not only biology but also our society. However, the exact distribution of totipotent cells in mammals remains elusive, although zygotes and single blastomeres at 2-cell stage embryos has been thought to be only mouse cells to be totipotent. We now show that a single blastomere isolated from 2- and 4-cell stage embryos gives rise to a fertile adult individual when it placed in a uterus, although isolation of blastomeres at these stage results in the disturbance of transcriptome in single blastomere derived embryos. Single blastomeres from 8-cell and morula stage embryos that were separately cultured in vitro exhibited severe defects in the formation of epiblast and primitive endoderm in the inner cell mass and the development to blastocysts, respectively. Our results thus indicate that totipotency of mouse zygotes extends to single blastomeres of 4-cell stage embryos.
Overall design Single-cell RNA sequencing of embryos with cavity derived from single blastomere of 1-, 2-, 4-, and 8-cell stage embryos.
Contributor(s) Maemura M, Taketsuru H, Nakajima Y, Shao R, Kakihara A, Nogami J, Ohkawa Y, Tsukada Y
Citation(s) 34045607
Submission date Dec 17, 2020
Last update date Jun 03, 2021
Contact name Yu-ichi Tsukada
Organization name Kyushu University
Department INAMORI Frontier Research Center Advanced Biological Information Research Division
Street address 744 Motooka, Fukuoka Nishi-ku
City Fukuoka
ZIP/Postal code 819-0395
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (2)
GSM4979207 Mix-1/8cell-Blasto
GSM4979208 Mix-2/4cell-Blasto
BioProject PRJNA686092
SRA SRP298343

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Supplementary file Size Download File type/resource
GSE163427_Blasto_umi_counts.txt.gz 3.4 Mb (ftp)(http) TXT
GSE163427_Mix_barcode.csv.gz 205 b (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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