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Series GSE162893 Query DataSets for GSE162893
Status Public on Jun 15, 2022
Title Maternal age affects D8 embryo gene expression both in trophoblast and inner cell
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary The aim of the study was to compare the effects of maternal age on embryos in horses. For that, 11 embryos were collected at 8 days post ovulation (expanded blastocyst stage) from 2 groups of multiparous mares of different age: 5 from young mares (<10 years old) and 6 from old mares (10-17 years old). Embryos collected were cut in 2 parts: one hemi-embryo containing pure trophoblast and the other one containing trophoblast + inner cell mass. ARN extractions were performed for all samples using PicoPure RNA isolation kit (Applied Biosystem). Five nanograms of both parts of each embryo were sequenced in paired-end with a length of 30-50bp separately using Illumina NextSeq 500 High. Data were trimmed using Cutadapt. Sequences with less than 10bp were removed.
Overall design 11 embryos cut in ICM and TE parts analyzed according to maternal age (Old or young mares)
Contributor(s) Derisoud E, Jouneau L, Dubois C, Archilla C, Jaszczyszyn Y, Legendre R, Daniel N, Peynot N, Dahirel M, Wimel L, Duranthon V, Chavatte-Palmer P
Citation(s) 35705916
Submission date Dec 08, 2020
Last update date Jun 23, 2022
Contact name Emilie Derisoud
Organization name INRAE
Street address 26 Rue des Prés,
State/province Essonne
ZIP/Postal code 91120
Country France
Platforms (1)
GPL21401 Illumina NextSeq 500 (Equus caballus)
Samples (22)
GSM4964785 OM, Embryo 1, ICM
GSM4964786 OM, Embryo 1, TE
GSM4964787 OM, Embryo 2, ICM
This SubSeries is part of SuperSeries:
GSE193676 EPAJ: Effect of mares' parity and age
BioProject PRJNA683651
SRA SRP297141

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Supplementary file Size Download File type/resource
GSE162893_XIST_IGV_SRY.gtf.gz 322 b (ftp)(http) GTF
GSE162893_XIST_IGV_stringtie.fasta.gz 6.4 Kb (ftp)(http) FASTA
GSE162893_counts_CH_OMvsYM.txt.gz 752.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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