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| Status |
Public on Jun 15, 2022 |
| Title |
Maternal age affects D8 embryo gene expression both in trophoblast and inner cell |
| Organism |
Equus caballus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of the study was to compare the effects of maternal age on embryos in horses. For that, 11 embryos were collected at 8 days post ovulation (expanded blastocyst stage) from 2 groups of multiparous mares of different age: 5 from young mares (<10 years old) and 6 from old mares (10-17 years old). Embryos collected were cut in 2 parts: one hemi-embryo containing pure trophoblast and the other one containing trophoblast + inner cell mass. ARN extractions were performed for all samples using PicoPure RNA isolation kit (Applied Biosystem). Five nanograms of both parts of each embryo were sequenced in paired-end with a length of 30-50bp separately using Illumina NextSeq 500 High. Data were trimmed using Cutadapt. Sequences with less than 10bp were removed.
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| Overall design |
11 embryos cut in ICM and TE parts analyzed according to maternal age (Old or young mares)
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| Contributor(s) |
Derisoud E, Jouneau L, Dubois C, Archilla C, Jaszczyszyn Y, Legendre R, Daniel N, Peynot N, Dahirel M, Wimel L, Duranthon V, Chavatte-Palmer P |
| Citation(s) |
35705916 |
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| Submission date |
Dec 08, 2020 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Emilie Derisoud |
| E-mail(s) |
emilie.derisoud@gmail.com, emilie.derisoud@inrae.fr
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| Organization name |
INRAE
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| Street address |
26 Rue des Prés,
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| City |
PALAISEAU |
| State/province |
Essonne |
| ZIP/Postal code |
91120 |
| Country |
France |
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| Platforms (1) |
| GPL21401 |
Illumina NextSeq 500 (Equus caballus) |
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| Samples (22)
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| GSM4964788 |
OM, Embryo 2, TE |
| GSM4964789 |
OM, Embryo 3, ICM |
| GSM4964790 |
OM, Embryo 3, TE |
| GSM4964791 |
OM, Embryo 4, ICM |
| GSM4964792 |
OM, Embryo 4, TE |
| GSM4964793 |
OM, Embryo 5, ICM |
| GSM4964794 |
OM, Embryo 5, TE |
| GSM4964795 |
OM, Embryo 6, ICM |
| GSM4964796 |
OM, Embryo 6, TE |
| GSM4964797 |
YM, Embryo 1, ICM |
| GSM4964798 |
YM, Embryo 1, TE |
| GSM4964799 |
YM, Embryo 2, ICM |
| GSM4964800 |
YM, Embryo 2, TE |
| GSM4964801 |
YM, Embryo 3, ICM |
| GSM4964802 |
YM, Embryo 3, TE |
| GSM4964803 |
YM, Embryo 4, ICM |
| GSM4964804 |
YM, Embryo 4, TE |
| GSM4964805 |
YM, Embryo 5, ICM |
| GSM4964806 |
YM, Embryo 5, TE |
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| This SubSeries is part of SuperSeries: |
| GSE193676 |
EPAJ: Effect of mares' parity and age |
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| Relations |
| BioProject |
PRJNA683651 |
| SRA |
SRP297141 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162893_XIST_IGV_SRY.gtf.gz |
322 b |
(ftp)(http) |
GTF |
| GSE162893_XIST_IGV_stringtie.fasta.gz |
6.4 Kb |
(ftp)(http) |
FASTA |
| GSE162893_counts_CH_OMvsYM.txt.gz |
752.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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