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| Status |
Public on Mar 03, 2021 |
| Title |
PRDM16 regulates a temporal transcriptional program to promote progression of cortical neural progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Radial glia (RG) in the neocortex sequentially generate distinct subtypes of projection neurons, accounting for the diversity and complex assembly of cortical neural circuits. Mechanisms that drive the rapid and precise temporal progression of RG are beginning to be elucidated. Here we reveal that the RG-specific transcriptional regulator PRDM16 promotes the transition of early to late phase of neurogenesis in the mouse neocortex. Loss of Prdm16 delays the timely progression of RG, leading to defective cortical laminar organization. Our genomic analyses demonstrate that PRDM16 regulates a subset of genes that are dynamically expressed between early and late neurogenesis. We show that PRDM16 suppresses target gene expression through limiting chromatin accessibility of permissive enhancers. We further confirm that critical target genes regulated by PRDM16 comprise neuronal specification genes, cell cycle regulators and molecules required for neuronal migration. These findings provide evidences to support that neural progenitors temporally shift gene expression program to achieve neural cell diversity.
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| Overall design |
To study the function of PRDM16 in gene regulation, ChIP-seq, RNA-seq and ATAC-seq datasets were generated to identify its genomic binding sites, gene expression change and chromatin accessibility change in the mutant versus wild-type control.
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| Contributor(s) |
Dai Q, Wen J |
| Citation(s) |
33597191 |
| |
| Submission date |
Dec 08, 2020 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Jiayu Wen |
| Organization name |
John Curtin School of Medical Research, The Australian National University
|
| Department |
Department of Genome Science
|
| Lab |
The Wen Lab
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| Street address |
133 Garran Rd
|
| City |
CANBERRA |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA683596 |
| SRA |
SRP297076 |
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