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| Status |
Public on Mar 18, 2021 |
| Title |
Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation [RNAseq_48hrs] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Negative Elongation Factor (NELF) is a critical transcriptional regulator that works through stabilizing paused RNA Polymerase to permit rapid gene expression changes in response to environmental cues. Whilst NELF is essential for embryonic development, its role in adult stem cells remains unclear. Here, through a muscle stem cell-specific deletion, we show that NELF is required for efficient muscle regeneration and replenishing the stem cell pool. Mechanistic studies using PRO-seq, single cell trajectory analyses and myofiber cultures revealed that NELF works at a specific stage of muscle regeneration whereby it mediates massive expansion of muscle progenitors. Strikingly, transplantation experiments indicate that these progenitors are also necessary for stem cell pool repopulation implying they are able to return to quiescence. Thus, we identified a critical role for NELF in expansion of muscle progenitors in response to injury and revealed that progenitors returning to quiescence are major contributors to stem cell pool repopulation.
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| Overall design |
RNA-seq was performed on myogenic progenitor cells which were FACS isolated from regenerating skeletal muscle 48-hours after skeletal muscle damage was induced with Cartiotoxin. Three replicates were performed for Wildtype (WT) populations, and three replicates performed for NELF-BscKO (KO) populations.
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| Contributor(s) |
Robinson DC, Ritso M, Nelson GM, Dilworth FJ |
| Citation(s) |
33735618, 34467227 |
| |
| Submission date |
Dec 07, 2020 |
| Last update date |
Dec 02, 2023 |
| Contact name |
Jeffrey Dilworth |
| E-mail(s) |
fdilworth@wisc.edu
|
| Phone |
6082653758
|
| Organization name |
University of Wisconsin
|
| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE150280 |
Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation |
|
| Relations |
| BioProject |
PRJNA683003 |
| SRA |
SRP296779 |
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