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| Status |
Public on May 21, 2010 |
| Title |
Transcriptional regulation of metabolism associated with desiccation resistance of the cactophilic Drosophila mojavensis |
| Organism |
Drosophila mojavensis |
| Experiment type |
Expression profiling by array
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| Summary |
In Drosophila, adaptation to xeric environments presents many challenges, greatest among them the maintenance of water balance. Drosophila mojavensis, a cactophilic species from the deserts of North America, is one of the most desiccation-resistant in the genus, surviving low humidity primarily by reducing its metabolic rate. Genetic control of reduced metabolic rate, however, has yet to be elucidated. We utilized the recently sequenced genome of D. mojavensis to create an oligonucleotide microarray in order to pursue the identities of the genes involved in metabolic regulation during desiccation. We observed large differences in gene expression between male and female D. mojavensis as well as both quantitative and qualitative sex differences in their ability to survive xeric conditions. As expected, genes associated with metabolic regulation and carbohydrate metabolism were differentially regulated between stress treatments. Most importantly, we identified four points in central metabolism (Glyceraldehyde 3-phosphate dehydrogenase, transaldolase, alcohol dehydrogenase and phosphoenolpyruvate carboxykinase) that indicate the potential mechanisms controlling metabolic rate reduction associated with desiccation resistance. Furthermore, a large number of genes associated with vision pathways also were differentially expressed between stress treatments, especially in females, that may underlie the initial detection of stressful environments and trigger subsequent metabolic changes.
Dataset from Transcriptional regulation of metabolism associated with the increased desiccation resistance of the cactophilic Drosophila mojavensis Matzkin,LM and Markow, MA, Genetics.
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| Overall design |
The stock used in this study (15081-1352.22) was the same one utilized for the recently published D. mojavensis genome sequence. Flies were reared using standard Tucson Drosophila Stock Center banana/Opuntia media. The experimental design consisted of two mating status treatments (virgin and mated) and two stress treatments (desiccation and food) for both sexes. There were two replicates per mating status/stress/sex treatment (16 total hybridizations)
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| Contributor(s) |
Matzkin LM, Markow TA |
| Citation(s) |
19487561 |
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| Submission date |
May 26, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Luciano Matias Matzkin |
| E-mail(s) |
lmm0015@uah.edu
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| Phone |
256-824-4326
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| Organization name |
University of Alabama in Huntsville
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| Department |
Biological Sciences
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| Street address |
301 Sparkman Drive
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| City |
Huntsville |
| State/province |
Alabama |
| ZIP/Postal code |
35899 |
| Country |
USA |
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| Platforms (1) |
| GPL8596 |
NimbleGen Matzkin Drosophila mojavensis 70K array v1.0. |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA115171 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16234_RAW.tar |
23.6 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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