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Status |
Public on Apr 29, 2021 |
Title |
Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens |
Organism |
Enterococcus faecalis |
Experiment type |
Expression profiling by array
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Summary |
We present evidence of mechanistically diverse allosteric relays converging on a few peptidyltransferase center (PTC) nucleotides, and propose a general model of antibiotic resistance mediated by these ARE-ABCFs
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Overall design |
E. faecalis cell lysate and RqcH 50s-bound (i.e. immunopreciptated, RqcH 50s-IP) tRNAs used for tRNA microarrays to measure tRNA abundance – 2 biological replicates in total; 2 – RqcH 50s-IP and 1 - total tRNA (lysate)
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Contributor(s) |
Takada H, Crowe-McAuliffe C, Polte C, Hairyliuk V, Wilson D, Ignatova Z |
Citation(s) |
34117249 |
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Submission date |
Nov 25, 2020 |
Last update date |
Jun 16, 2021 |
Contact name |
Zoya Ignatova |
E-mail(s) |
zoya.ignatova@chemie.uni-hamburg.de
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Organization name |
Universitaet Hamburg
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Street address |
Martin -Luther-King-Platz 6
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City |
Hamburg |
ZIP/Postal code |
20146 |
Country |
Germany |
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Platforms (1) |
GPL29446 |
Microarrays of Enterococcus faecalis encoded tRNAs |
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Samples (2) |
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Relations |
BioProject |
PRJNA680786 |