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| Status |
Public on Apr 29, 2021 |
| Title |
Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens |
| Organism |
Enterococcus faecalis |
| Experiment type |
Expression profiling by array
|
| Summary |
We present evidence of mechanistically diverse allosteric relays converging on a few peptidyltransferase center (PTC) nucleotides, and propose a general model of antibiotic resistance mediated by these ARE-ABCFs
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| Overall design |
E. faecalis cell lysate and RqcH 50s-bound (i.e. immunopreciptated, RqcH 50s-IP) tRNAs used for tRNA microarrays to measure tRNA abundance – 2 biological replicates in total; 2 – RqcH 50s-IP and 1 - total tRNA (lysate)
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| Contributor(s) |
Takada H, Crowe-McAuliffe C, Polte C, Hairyliuk V, Wilson D, Ignatova Z |
| Citation(s) |
34117249 |
| |
| Submission date |
Nov 25, 2020 |
| Last update date |
Jun 16, 2021 |
| Contact name |
Zoya Ignatova |
| E-mail(s) |
zoya.ignatova@chemie.uni-hamburg.de
|
| Organization name |
Universitaet Hamburg
|
| Street address |
Martin -Luther-King-Platz 6
|
| City |
Hamburg |
| ZIP/Postal code |
20146 |
| Country |
Germany |
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| Platforms (1) |
| GPL29446 |
Microarrays of Enterococcus faecalis encoded tRNAs |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA680786 |
