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| Status |
Public on Dec 23, 2020 |
| Title |
Low avidity T cell responses to SARS-CoV-2 in unexposed individuals and severe COVID-19 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pre-existing memory T-cells against SARS-CoV-2 are present in a fraction of unexposed individuals and their induction by common cold corona viruses (CCCoVs) infection is suggested. Here we demonstrate that SARS-CoV-2-reactive T-cells were present in the memory compartment of virtually all unexposed individuals but possessed only low functional avidity. They harbored multiple, highly variable cross-reactivities that were not restricted to CCCoVs. Cross-reactivity to CCCoV was almost absent in COVID-19 patients. This was irrespective of strong T-cell memory against CCCoV in all donors. In severe but not mild COVID-19, SARS-CoV-2-specific T-cells also displayed low functional avidity and reduced clonal expansion, despite strongly increased frequencies. Our data question a major protective role of CCCoV for COVID-19. Instead, we suggest that a low avidity pre-existing T-cell memory may contribute to the excessive but low avidity T-cell responses, which we identified here as a hallmark of severe COVID-19.
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| Overall design |
Single-cell sequencing of 6 unexposed and 14 COVID-19 patients
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| Contributor(s) |
Bacher P, Rosati E, Esser D, Rios Martini G, Saggau C, Schiminsky E, Dargvainiene J, Schröder I, Wieters I, Khodamoradi Y, Eberhardt F, Vehreschild MJ, Neb H, Sonntagbauer M, Conrad C, Tran F, Rosenstiel P, Markewitz R, Wandinger K, Augustin M, Rybniker J, Kochanek M, Leypoldt F, Cornely OA, Koehler P, Franke A, Scheffold A |
| Citation(s) |
33296686 |
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| Submission date |
Nov 24, 2020 |
| Last update date |
Dec 23, 2020 |
| Contact name |
Daniela Esser |
| Organization name |
University Hospital Schleswig-Holstein
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| Department |
Institute of Clinical Chemistry
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| Lab |
Neuroimmunology
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| Street address |
Arnold-Heller-Str. 3
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| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA680539 |
| SRA |
SRP293741 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162086_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
| GSE162086_seurat_metadata.tsv.gz |
5.7 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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