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| Status |
Public on May 19, 2021 |
| Title |
RNAseq and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16 |
| Organism |
Dictyostelium discoideum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Global survey of the transcriptome and comparison to the proteome in autophagy-deficient strains of D. discoideum. Results: Our RNAseq analysis of ATG9‾, ATG16‾ and ATG9‾/16‾ cells revealed major transcriptional changes in a large number of genes in comparison to AX2. Conclusions: Our study provides the first combined transcriptome and proteome analysis of ATG9‾, ATG16‾ and ATG9‾/16‾ cells and adds to our knowledge of functions of these proteins in Dictyostelium discoideum.
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| Overall design |
Total RNA from vegetative Dictyostelium discoideum cells (AX2 wild-type and ATG9‾, ATG16‾, ATG9‾/16‾ mutant strains) was isolated; sequencing and analysis was as described in Karow et al. Cells, 9(5), E1179. n=9, Illumina Genome Analyzer IIx
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| Contributor(s) |
Xiong Q, Song N, LI P, Fischer S, Konertz R, Wagle P, Glöckner G, Wu C, Eichinger L |
| Citation(s) |
34126926 |
| |
| Submission date |
Nov 24, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
Ludwig Eichinger |
| E-mail(s) |
ludwig.eichinger@uni-koeln.de
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| Phone |
+49 221 478 6928
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| Organization name |
Centre for Biochemistry Medical Faculty University of Cologne
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| Department |
Biochemistry Medical Faculty
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| Lab |
Cellular Homeostasis
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| Street address |
Joseph-Stelzmann-Str. 52
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| City |
Cologne |
| State/province |
NRW |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL15643 |
Illumina HiSeq 2000 (Dictyostelium discoideum) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA680492 |
| SRA |
SRP293794 |
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