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| Status |
Public on Nov 20, 2009 |
| Title |
Loss of Syk in normal breast cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Loss of Syk in normal breast cells in vivo and in vitro: gene expression and phenotypic switch to stem-cell like with induction of invadopodia
Morphological and gene microarray analysis following Syk knockdown in MCF10A reveals a loss of luminal and differentiated epithelial features with epithelial to mesenchymal transition and a gain in invadopodia and stem/ progenitor cell marker expression.
These results support the role of Syk in limiting normal stem/progenitor proliferation and invasion during morphogenesis, and support the role of Syk as a critical tumor suppressor for breast cancer.
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| Overall design |
Global gene expression profiles were measured using Affymetrix U133A 2.0 microarrays in MCF10A cell lines (control and Syk siRNA knockdown cells on collagen gels or plastic).
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| Contributor(s) |
Sung YM, Xu X, Sun J, Mueller D, Sentissi K, Johnson P, Urbach E, Seillier-Moiseiwitsch F, Johnson M, Mueller SC |
| Citation(s) |
19829710 |
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| Submission date |
May 22, 2009 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Junfeng Sun |
| Organization name |
Georgetown Univ
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| Street address |
Suite 180, 4000 Reservior Rd NW
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| GSM406563 |
Empty vector, plastic plate, rep2 |
| GSM406564 |
Empty vector, collagen plate, rep3 |
| GSM406565 |
Empty vector, plastic plate, rep3 |
| GSM406566 |
siRNA, collagen plate, rep1 |
| GSM406567 |
siRNA, plastic plate, rep1 |
| GSM406568 |
siRNA, collagen plate, rep2 |
| GSM406569 |
siRNA, plastic plate, rep2 |
| GSM406570 |
siRNA, collagen plate, rep3 |
| GSM406571 |
siRNA, plastic plate, rep3 |
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| Relations |
| BioProject |
PRJNA117139 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16200_RAW.tar |
24.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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