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Series GSE16200 Query DataSets for GSE16200
Status Public on Nov 20, 2009
Title Loss of Syk in normal breast cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Loss of Syk in normal breast cells in vivo and in vitro: gene expression and phenotypic switch to stem-cell like with induction of invadopodia

Morphological and gene microarray analysis following Syk knockdown in MCF10A reveals a loss of luminal and differentiated epithelial features with epithelial to mesenchymal transition and a gain in invadopodia and stem/ progenitor cell marker expression.

These results support the role of Syk in limiting normal stem/progenitor proliferation and invasion during morphogenesis, and support the role of Syk as a critical tumor suppressor for breast cancer.
Overall design Global gene expression profiles were measured using Affymetrix U133A 2.0 microarrays in MCF10A cell lines (control and Syk siRNA knockdown cells on collagen gels or plastic).
Contributor(s) Sung YM, Xu X, Sun J, Mueller D, Sentissi K, Johnson P, Urbach E, Seillier-Moiseiwitsch F, Johnson M, Mueller SC
Citation(s) 19829710
Submission date May 22, 2009
Last update date Dec 06, 2018
Contact name Junfeng Sun
Organization name Georgetown Univ
Street address Suite 180, 4000 Reservior Rd NW
City Washington
State/province DC
ZIP/Postal code 20057
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM406560 Empty vector, collagen plate, rep1
GSM406561 Empty vector, plastic plate, rep1
GSM406562 Empty vector, collagen plate, rep2
BioProject PRJNA117139

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Supplementary file Size Download File type/resource
GSE16200_RAW.tar 24.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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