|
| Status |
Public on Nov 29, 2020 |
| Title |
Expression profiling with RNA-seq between mTORC1 high and low AML cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We reported the expression profiling between mTORC1 high and low mouse AML cells with MLL-AF9 utilizing a novel fluorescent probe named mVenus-TOSI (TOr-Signal-Indicator). For this purpose, 3 mTOR high (mVenus low) and 4 mTOR low (mVenus high) Mouse AML cells were harvested from 4 mice. The transcriptomic profiles were distinctive and gene ontology analysis revealed that mTORC1 activity was associated with differentially expressed immune response and metabolism related genes. Further, gene signature enrichment analysis (GSEA) validated that the mTORC1 signal signature correlated with mTORC1 activity and that leukemia stem cell (LSC), Myc, and cell cycle related signatures were enriched in mTORC1 high cells.
|
| |
|
| Overall design |
Examining expression profilng between 3 mTORC1 high and 4 mTORC1 low AML cells with RNA-seq
|
| |
|
| Contributor(s) |
Oki T, Kato H, Mercier F, Scadden D |
| Citation(s) |
33431855 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA194596 |
(PQC2) Localization as a determinant of cancer dormancy |
MASSACHUSETTS GENERAL HOSPITAL |
David T Scadden |
| U54 CA193461 |
Evolution and Treatment Response of Brain, Breast, and Hematologic Malignancies |
DANA-FARBER CANCER INSTITUTE |
Franziska Michor |
| R01 CA194596 |
(PQC2) Localization as a determinant of cancer dormancy |
MASSACHUSETTS GENERAL HOSPITAL |
Charles P. Lin |
|
| |
| Submission date |
Nov 12, 2020 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Toshihiko Oki |
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA677958 |
| SRA |
SRP292361 |
Less...
More...