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Series GSE160934 Query DataSets for GSE160934
Status Public on Feb 26, 2021
Title Nucleo-cytoplasmic RNA distribution responsible for maintaining neuroinflammatory microenvironment.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Characteristics of subcellular localization of RNAs are closely associated with their transcriptional outputs and, therefore, involved in the physiological process of many cases of central nervous system diseases, such as hemorrhagic stroke, which threatens millions of people and causes of death and long-term disability world-wide.  Using whole-transcriptome sequencing (RNA-seq), here we report a subcellular gene profiling during hemorrhagic damage. Differentially expressed genes owing to spatial distribution of oxyhemoglobin (oxy-hb) treated-microglial nuclear and cytoplasmic fractions were enriched in biological processes in RNA metabolism and RNA splicing regardless of the duration of stimulation; while hemorrhage-induced differentially expressed genes played roles in inflammation regardless of the duration of stimulation. Interestingly, nuclear-retained intron of microglial nucleocytoplasmic transport genes may result in their down-regulation in 24 hours of oxy-Hb stimulation. This transcriptome study advances our understanding of the contributor of subcellular components, revealing new insights into cytoplasmic non-coding RNAs in response to hemorrhage and provide a resource for investigating comparable diseases.
Overall design BV2 mouse microgial cells were treated with 10μM oxyhemoglobin for 0, 12 or 24 hours and harvested to produce nuclear and cytoplasmic RNA samples
Contributor(s) Liao Y, Kuang C, Bao Z
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Submission date Nov 05, 2020
Last update date Feb 27, 2021
Contact name Zheng Bao
Organization name The Affiliated Hospital of Southwest Medical University
Street address No. 25, Taiping Street
City Luzhou
State/province Sichuan
ZIP/Postal code 646000
Country China
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (18)
GSM4886719 microgila BV2 cells-nuclear fraction_control_rep1
GSM4886720 microgila BV2 cells-nuclear fraction_control_rep2
GSM4886721 microgila BV2 cells-nuclear fraction_control_rep3
BioProject PRJNA674859
SRA SRP291327

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE160934_BV2-Control-Cytoplasmic.tpm.txt.gz 300.0 Kb (ftp)(http) TXT
GSE160934_BV2-Control-Nuclear.tpm.txt.gz 328.7 Kb (ftp)(http) TXT
GSE160934_BV2-Hb-12h-Cytoplasmic.tpm.txt.gz 301.6 Kb (ftp)(http) TXT
GSE160934_BV2-Hb-12h-Nuclear.tpm.txt.gz 330.6 Kb (ftp)(http) TXT
GSE160934_BV2-Hb-24h-Cytoplasmic.tpm.txt.gz 307.9 Kb (ftp)(http) TXT
GSE160934_BV2-Hb-24h-Nuclear.tpm.txt.gz 332.1 Kb (ftp)(http) TXT
GSE160934_Raw_gene_counts_matrix.txt.gz 457.6 Kb (ftp)(http) TXT
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