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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 13, 2020 |
| Title |
Age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 in lung epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The SARS-CoV-2 novel coronavirus global pandemic (COVID-19) has led to millions of cases and hundreds of thousands of deaths globally. While older adults appear at high risk for severe disease, hospitalizations and deaths due to SARS-CoV-2 among children have been relatively rare. Integrating single-cell RNA sequencing (scRNA-seq) of developing mouse lung with temporally-resolved immunofluorescence in mouse and human lung tissue, we found expression of SARS-CoV-2 Spike protein primer TMPRSS2 was highest in ciliated cells and type I alveolar epithelial cells (AT1), and TMPRSS2 expression increased with aging in mice and humans. Analysis of autopsy tissue from fatal COVID-19 cases detected SARS-CoV-2 RNA most frequently in ciliated and secretory cells in airway epithelium and AT1 cells in peripheral lung. SARS-CoV-2 RNA was highly colocalized in cells expressing TMPRSS2. Together, these data demonstrate the cellular spectrum infected by SARS-CoV-2 in lung epithelium and suggest that developmental regulation of TMPRSS2 may underlie the relative protection of infants and children from severe respiratory illness.
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| Overall design |
Single Cell RNAseq of dissociated mouse lungs at ages: E18, P0, P7, P14, and P64. Suspensions were enriched for cells that were CD45 negative, Ter119 negative, and viable.
These data are part of a larger investigation (data not provided here) examining age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 in lung epithelium.
[contributor] Vanderbilt COVID-19 Consortium Cohort
[contributor] Human Cell Atlas Biological Network
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| Contributor(s) |
Schuler BA, Habermann AC, Plosa EJ, Taylor CJ, Jetter C, Negretti NM, Kapp ME, Benjamin JT, Gulleman P, Nichols DS, Braunstein LZ, Hackett A, Koval M, Guttentag SH, Blackwell TS, Webber SA, Banovich NE, Kropski JA, Sucre JM |
| Citation(s) |
33180746 |
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| Submission date |
Nov 05, 2020 |
| Last update date |
Nov 15, 2020 |
| Contact name |
Jennifer Sucre |
| Organization name |
Vanderbilt University Medical Center
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| Department |
Division of Neonatology, Department of Pediatrics
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| Street address |
2215 B Garland Ave, 1125 Light Hall
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA674755 |
| SRA |
SRP291263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160876_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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