|
| Status |
Public on Nov 04, 2020 |
| Title |
A temporally resolved transcriptome for developing “Keller” explants of the Xenopus laevis dorsal marginal zone |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Explanted tissues from vertebrate embryos reliably develop in culture and have provided essential paradigms for understanding embryogenesis, from early embryological investigations of induction, to the extensive study of Xenopus animal caps, to the current studies of mammalian gastruloids. Cultured explants of the Xenopus dorsal marginal zone (“Keller” explants) serve as a central paradigm for studies of convergent extension cell movements, yet we know little about the global patterns of gene expression in these explants. In an effort to more thoroughly develop this important model system, we provide here a time-resolved bulk transcriptome for developing Keller explants.
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| |
|
| Overall design |
RNA-Seq analysis of a timecourse of dorsal marginal zone explants from Xenopus embryos from stage 11 to stage 18.
|
| |
|
| Contributor(s) |
Kakebeenm A, Huebner R, Shindo A, Kwon K, Kwon T, Wills A, Wallingford J |
| Citation(s) |
33368695 |
| |
| Submission date |
Nov 03, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Anneke Dixie Kakebeen |
| E-mail(s) |
akakebee@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Biochemistry
|
| Lab |
Andrea Wills
|
| Street address |
5611 9th Ave NW
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98107 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA674389 |
| SRA |
SRP291031 |
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