 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 15, 2021 |
| Title |
Post-translational modification of Sox11 regulates RGC survival and axon regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The failure of adult CNS neurons to survive and regenerate their axons after injury or in neurodegenerative disease remains a major target for basic and clinical neuroscience. Recent data demonstrated in the adult mouse that exogenous expression of Sry-related high-mobility-box 11 (Sox11) promotes optic nerve regeneration after optic nerve injury, but exacerbates the death of a subset of retinal ganglion cells, alpha-RGCs. During development, Sox11 is required for RGC differentiation from retinal progenitor cells (RPCs), and we found that mutation of a single residue to prevent sumoylation at K91 increased nuclear localization and RGC differentiation in vitro. Here we explored whether this Sox11 manipulation similarly has stronger effects on RGC survival and optic nerve regeneration. In vitro, we found that non-SUMOylatable Sox11 K91A leads to RGC death and suppresses axon outgrowth in primary neurons. We furthermore found that Sox11 K91A more strongly promotes axon regeneration but also increases RGC death after optic nerve injury in vivo in adult mouse. RNA-seq data showed that Sox11 and Sox11 K91A increase the expression of key signaling pathway genes associated with axon growth and regeneration but downregulated Spp1 and Opn4 expression in RGC cultures, consistent with negatively regulating the survival of α-RGCs and ipRGCs. Thus Sox11 and its sumoylation site at K91 regulate gene expression, survival and axon growth in RGCs and may be explored further as potential regenerative therapies for optic neuropathy.
|
| |
|
| Overall design |
The changes in the gene expression profiles were investigated by RNA-seq
|
| |
|
| Contributor(s) |
Chang K, Madaan A, Tanasa B, Goldberg J |
| Citation(s) |
33441400 |
| Submission date |
Nov 02, 2020 |
| Last update date |
Jan 15, 2021 |
| Contact name |
Jeffrey L Goldberg |
| E-mail(s) |
jlgoldbe@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Opthalmology
|
| Lab |
Goldberg
|
| Street address |
1651 Page Mill Road
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
| GSM4876165 |
RGC that have been transduced with CONTROL, replicate1 |
| GSM4876166 |
RGC that have been transduced with CONTROL, replicate2 |
| GSM4876167 |
RGC that have been transduced with SOX11_WT, replicate1 |
| GSM4876168 |
RGC that have been transduced with SOX11_WT, replicate2 |
| GSM4876169 |
RGC that have been transduced with SOX11_MUTANT, replicate1 |
| GSM4876170 |
RGC that have been transduced with SOX11_MUTANT, replicate2 |
|
| Relations |
| BioProject |
PRJNA673813 |
| SRA |
SRP290800 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160627_RAW.tar |
13.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...