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Series GSE16053 Query DataSets for GSE16053
Status Public on Oct 13, 2009
Title miR-17 seed family specific targets in two Hodgkin lymphoma cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of the study is to identify miRNA specific targets in Hodgkin lymphoma cell lines. By immunoprecipitation (IP) of wild type Ago2, Ago2 associated gene transcripts (ie miRNA targets) are coimmunoprecipitated. In cells transfected with anti-miR-17/20/93/106, the miR-17 seed family specific targets are not coimmunoprecipitated with Ago2. With microarray analysis, signal intensities of probes associated with Ago2 from untransfected and anti-miRNA transfected cells are compared. Gene transcripts that are depleted from the Ago2-IP fraction upon miRNA inhibition (ie miRNA specific targets) are identified.
Overall design RNA isolated from the Ago2 immunoprecipitated (IP) fraction is hybridized against the RNA from total cell lysate (T) fraction. The experiment is performed in two independent Hodgkin lymphoma cell lines, L1236 and L428. Samples included anti-miR-220 transfected L428, anti-miR-17/20/93/106 transfected L428 and anti-miR-17/20/93/106 transfected L1236. All hybridizations are performed with a dye swap design.
Contributor(s) Tan LP, Seinen E, Duns G, de Jong D, Sibon OC, Kok K, Kroesen B, Poppema S, van den Berg A
Citation(s) 19734348
Submission date May 11, 2009
Last update date Feb 22, 2018
Contact name Lu Ping Tan
Phone 31503617085
Organization name University Medical Center Groningen
Department Department of Pathology
Street address Hanzeplein 1
City Groningen
ZIP/Postal code 9711Rb
Country Netherlands
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (6)
GSM402139 L1236 a17IP/a17T
GSM402140 L1236 a17T/a17IP
GSM402141 L428 a17IP/a17T
BioProject PRJNA114997

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16053_RAW.tar 88.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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