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| Status |
Public on Oct 30, 2020 |
| Title |
Chromosomal loop engineering in human beta globin locus (CUT & RUN) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We have engineered the 3 prime HS1 CTCF binding site at the human beta globin gene locus in HUDEP-2 cells and performed RNA-seq/ChIP-seq/ATAC-seq as well as HiC and capture HiC to dissect its potential in regulating gene expression
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| Overall design |
We have deleted 3 prime HS1 CTCF binding site in HUDEP-2 cells with CRISPR/Cas9 and inverted 3 prime HS1 CTCF binding site with CRISPR/Cas9-mediated HDR. In each condition, we have obtained two independent clones for the 3’HS1 CTCF binding site deletion and the 3 prime HS1 CTCF binding site inversion.
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| |
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| Contributor(s) |
Zhang X, Himadewi P, Gore H |
| Citation(s) |
34585664 |
| |
| Submission date |
Oct 29, 2020 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Xiaotian Zhang |
| E-mail(s) |
zxtzhangqian@gmail.com
|
| Organization name |
The University of Texas Health Science Center at Houston
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Zhang Lab
|
| Street address |
6431 Fannin St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (2) |
| GSM4873125 |
3 prime HS1 deleted HUDEP2 clone B6 CTCF Cut&Run |
| GSM4873126 |
3 prime HS1 inverted HUDEP2 clone A2 CTCF Cut&Run |
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| This SubSeries is part of SuperSeries: |
| GSE160425 |
Chromosomal loop engineering in human beta globin locus |
|
| Relations |
| BioProject |
PRJNA673302 |
| SRA |
SRP290310 |
