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| Status |
Public on Dec 20, 2020 |
| Title |
Semiochemical responsive olfactory sensory neurons are sexually dimorphic and plastic [RNA-Seq of whole olfactory mucosa] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding how genes and experiences work in concert to generate phenotypic variability will provide a better understanding of individuality. Here, we considered this in the context of the main olfactory epithelium, a chemosensory structure with over a thousand distinct cell types, in mice. We identified a subpopulation of at least three types of olfactory sensory neurons, defined by receptor expression, whose abundances were sexually dimorphic. This subpopulation of olfactory sensory neurons was over-represented in sex-separated female mice and responded robustly to the male-specific semiochemicals 2-sec-butyl-4,5-dihydrothiazole and (methylthio)methanethiol. Sex-combined housing led to a robust attenuation of the female over-representation. Testing of Bax-/- mice revealed a Bax-dependence in generating the sexual dimorphism in sex-separated mice. Altogether, our results suggest a profound role of experience and activity in influencing homeostatic mechanisms to generate a robust sexually dimorphic phenotype in the main olfactory epithelium.
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| Overall design |
RNA-Seq of whole olfactory mucosa from sex-separated and sex-combined male and female mice.
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| Contributor(s) |
Vihani A, Matsunami H |
| Citation(s) |
33231170 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DC014423 |
Peripheral Odor Coding in Mammals |
DUKE UNIVERSITY |
Hiroaki Matsunami |
| R01 DC016224 |
Biogenesis of olfactory G protein-coupled receptors |
DUKE UNIVERSITY |
Hiroaki Matsunami |
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| |
| Submission date |
Oct 28, 2020 |
| Last update date |
Dec 20, 2020 |
| Contact name |
Aashutosh Vihani |
| Organization name |
Duke University
|
| Street address |
264 CARL Building
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE160272 |
Semiochemical responsive olfactory sensory neurons are sexually dimorphic and plastic |
|
| Relations |
| BioProject |
PRJNA672853 |
| SRA |
SRP289424 |
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