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Series GSE159938 Query DataSets for GSE159938
Status Public on Feb 19, 2021
Title Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [bulk RNA-seq]
Organisms Acomys cahirinus; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue regeneration is a process that recapitulates the molecular and mechanical aspects of development and evolution. We use the wound-induced hair neogenesis (WIHN) model to investigate the mechanical and molecular responses of the laboratory (Mus) and African spiny (Acomys) mice. Laboratory and spiny mice showed an opposite trend of spatiotemporal morphogenetic field for WIHN during wound healing, and wound stiffness gradient across the whole wound bed predicated pattern of hair formation. Using bulk and single-cell RNA-seq analysis and K14-Cre-Twist1 transgenic mice, we identified the central role of the Twist1 pathway as the mediator of epidermal-dermal interaction and the emergence of periodic hair primordia. Lastly, we generated a Turing model with an underlying measure of stiffness to support a two-scale tissue mechanic model to explain the setup of a morphogenetic field from a wound bed (mm scale) or periodically arranged hair primordia from a morphogenetic field (μm scale). Delineating the common and distinct chemo-mechanical events during regenerative wound healing between laboratory and African spiny mice reveal its evo-devo advantages, which provide new perspectives for regenerative medicine.
Overall design bulk RNA-Seq for epidermal and dermal tissues from lab mouse and spiny mouse
Contributor(s) Chuong C, Harn HI
Citation(s) 33972536
Submission date Oct 23, 2020
Last update date May 19, 2021
Contact name Hans I-Chen Harn
Organization name University of Southern California
Street address 2011 Zonal Ave. HMR304
City Los Angeles
State/province CA
ZIP/Postal code 90089
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24885 Illumina NextSeq 500 (Acomys cahirinus)
Samples (25)
GSM4850917 Chuong806: Mm WT dorsal epidermis RNA-seq
GSM4850918 Chuong808: Mm WT dorsal epidermis RNA-seq
GSM4850919 Chuong812: Mm WT dorsal epidermis RNA-seq
This SubSeries is part of SuperSeries:
GSE159939 Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration
BioProject PRJNA670922
SRA SRP288318

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159938_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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