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Series GSE15991 Query DataSets for GSE15991
Status Public on May 06, 2009
Title Expression profile analysis of inflammatory response regulated by hepatocyte nuclear factor 4α
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To obtain a genomic view of hepatocyte nuclear factor-4α (HNF-4α) in the regulation of the inflammatory response, microarray analysis was used to probe the expression profile of an inflammatory response induced by cytokines in a model of knock-down HNF-4α HepG2 cells. The results indicate an extensive role for HNF-4α plays in the regulation of a large number of the liver-specific genes. Majority of genes (71%) affected by cytokine treatment are also affected by HNF-4α knock-down. This significant overlap suggests that HNF-4α may play a role in regulating the cytokine-induced inflammatory response.
Overall design The different treated HepG2 cells were grouped into 4 groups (four replicates in each group): Group1, Control; Group2, HNF-4α shRNA treated cells; Group3, cytokine treated group; Group4, HNF-4α shRNA and cytokine treatments. RNA extraction and hybridization on Affymetrix microarrays were processed. The expression profiles between different groups were analyzed and compared. In order to explore the function of HNF-4α in the inflammatory response, a set of 170 genes annotated as inflammatory response was obtained from GO (, the enrichments of these inflammatory genes were analyzed in different treated groups.
Contributor(s) Wang Z, Bishop EP, Burke PA
Citation(s) 21352552
Submission date May 06, 2009
Last update date Dec 06, 2018
Contact name Peter A Burke
Phone 617-414-8056
Fax 617-414-7398
Organization name Boston University School of Medicine
Department Surgery
Lab Trauma
Street address 850 Harrison Avenue, Dowling 2 South
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM400374 control 1
GSM400375 control 2
GSM400376 control 3
BioProject PRJNA115349

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Supplementary file Size Download File type/resource
GSE15991_RAW.tar 33.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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