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| Status |
Public on Dec 05, 2020 |
| Title |
Next Generation Sequencing of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. The goals of this study are to analysis the different mRNA expression between transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. Quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. We performed mRNA-seq in the NEAT1_2 knockdown group and NC group in the K1 cell line. We found that after knockdown of NEAT1_2, 615 mRNAs were upregulated and 2364 mRNAs were downregulated.
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| |
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| Overall design |
K1 cell lines mRNA profiles of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells
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| |
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| Contributor(s) |
Sun W, Qin Y, Zhang H |
| Citation(s) |
33738254 |
| |
| Submission date |
Oct 23, 2020 |
| Last update date |
Mar 23, 2021 |
| Contact name |
WEI SUN |
| E-mail(s) |
SUN19890208@126.COM
|
| Phone |
02483282191
|
| Organization name |
the First Hospital of China Medical University
|
| Street address |
No.155 Nanjing Bei Street
|
| City |
Shenyang |
| State/province |
Liaoning |
| ZIP/Postal code |
110001 |
| Country |
China |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA670856 |
| SRA |
SRP288273 |
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