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| Status |
Public on Oct 23, 2023 |
| Title |
Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Tissue regeneration is a process that recapitulates the molecular and mechanical aspects of development and evolution. We use the wound-induced hair neogenesis (WIHN) model to investigate the mechanical and molecular responses of the laboratory (Mus) and African spiny (Acomys) mice. Laboratory and spiny mice showed an opposite trend of spatiotemporal morphogenetic field for WIHN during wound healing, and wound stiffness gradient across the whole wound bed predicated pattern of hair formation. Using bulk and single-cell RNA-seq analysis and K14-Cre-Twist1 transgenic mice, we identified the central role of the Twist1 pathway as the mediator of epidermal-dermal interaction and the emergence of periodic hair primordia. Lastly, we generated a Turing model with an underlying measure of stiffness to support a two-scale tissue mechanic model to explain the setup of a morphogenetic field from a wound bed (mm scale) or periodically arranged hair primordia from a morphogenetic field (μm scale). Delineating the common and distinct chemo-mechanical events during regenerative wound healing between laboratory and African spiny mice reveal its evo-devo advantages, which provide new perspectives for regenerative medicine.
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| Overall design |
Single-cell mRNA sequencing for back skin from naïve mice using Drop-Seq
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| Contributor(s) |
Harn HI |
| Citation(s) |
33972536 |
| Submission date |
Oct 22, 2020 |
| Last update date |
Jan 22, 2024 |
| Contact name |
Hans I-Chen Harn |
| E-mail(s) |
hharn@usc.edu
|
| Organization name |
University of Southern California
|
| Street address |
2011 Zonal Ave. HMR304
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE159939 |
Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration |
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| Relations |
| BioProject |
PRJNA670657 |
| SRA |
SRP288285 |
