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| Status |
Public on Oct 09, 2020 |
| Title |
Gene expression profile of DOCK8+CD4 T cell which causes systemic lupus erythematosus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease of unknown cause. We show here that a novel T follicular helper cell type expressing the guanine nucleotide exchange factor DOCK8 on the cell surface causes SLE. These cells, which we have designated autoantibody-inducing CD4 T (aiCD4 T) cells, are generated after resuscitation from anergy following strong TCR stimulation by antigen. When mice normally not prone to autoimmune disease were repeatedly immunized with an antigen such as OVA, they generated DOCK8+ CD4 T cells. These DOCK8+ CD4 T cells, in vivo and also upon transfer to naïve mice, induced a variety of autoantibodies and lesions characteristic of SLE. TCR repertoire analyses showed that a substantial number of novel TCR repertoires were generated in the DOCK8+ CD4 T cells, which induced novel autoantibodies upon transfer to naïve mice. DOCK8+ CD4 T cells are localized in splenic red pulp, the space immunoreactive against a variety of antigens, and specifically increased in the peripheral blood of SLE patients in association with disease activity. Anti-DOCK8 antibody treatment ameliorated the lesions induced by DOCK8+ CD4 T cells and in lupus model (NZB x W) F1 mice. Thus, when CD4 T cells are overstimulated by an external disturbance, i.e., repeatedly stimulated with antigen, to levels that surpass the system’s self-organized criticality, these cells express DOCK8 on the cell surface and acquire autoreactivity via TCR re-revision at the periphery. These DOCK8+ CD4 T cells subsequently induce a variety of autoantibodies and SLE.
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| Overall design |
Gene expression in CD4 T cell of control mice immunized with PBS, DOCK8- and DOCK8+ CD4 T cell of mice which developed SLE by repeated (12x) immunization with ovalbumin (OVA) was mesured.
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| Contributor(s) |
Tsumiyama K, Shiozawa S |
| Citation(s) |
34977502 |
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| Submission date |
Oct 08, 2020 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Shunichi Shiozawa |
| E-mail(s) |
shiozawa@port.kobe-u.ac.jp
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| Organization name |
Institute for Rheumatic Diseases
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| Street address |
944-25 Fujita
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| City |
Kato |
| ZIP/Postal code |
673-1462 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA668120 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159240_RAW.tar |
86.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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