|
| Status |
Public on Jan 11, 2022 |
| Title |
Expression data from parental and evolved Saccharomyces cerevisiae F12 cells in the presence of insoluble solids at high concentration and lignocellulose-derived inhibitors |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Increasing yeast robustness against biomass-derived inhibitors and insoluble solids is essential for the realization of a bio-based economy. The xylose-fermenting Saccharomyces cereivisiae F12 strain was subjected to an adaptive laboratory evolution experiment in the presence of these stressors. The resulting evolved strain exhibit better fermentation performance in terms of xylose consumption and ethanol yields than the parental strain. The overexpression of genes related to cell wall integrity and the stress response, together with the downregulation of protein synthesis and iron transport and homeostasis were highlighted as the major biological processes responsible for the improved phenotype.
|
| |
|
| Overall design |
Parental and evolved S. cerevisiae F12 cells were collected during fermentation in presence of insoluble solids and lignocellulose-derived inhibitors for RNA extraction and hybridization on Affymetrix microarrays.
|
| Web link |
https://www.nature.com/articles/s41598-021-04554-4
|
| |
|
| Contributor(s) |
Moreno AD, Tomás-Pejó E |
| Citation(s) |
35017613 |
| |
| Submission date |
Oct 07, 2020 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Antonio David Moreno |
| E-mail(s) |
david.moreno@ciemat.es
|
| Phone |
+34 91 346 6054
|
| Organization name |
CIEMAT
|
| Department |
Department of Energy
|
| Lab |
Biofuels Unit
|
| Street address |
Avda. Complutense 40
|
| City |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA668011 |
Less...
More...