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| Status |
Public on Jul 30, 2021 |
| Title |
A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3'-processing and termination [NET-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
In this study, we reveal that BRD4 underlies a general 5'-elongation checkpoint that primes transcribing RNA polymerase II for 3'-RNA processing and transcription termination. BRD4-specific degradation impairs Pol II pause release, induces massive readthrough transcription, and RNA cleavage defects. Acute loss of BRD4 disrupts the recruitment of 3'-RNA processing factors.
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| Overall design |
SI-NET-seq measurements for indicated time points upon DMSO (control), dTAG7 (BRD4 degradation) and dBET6 (pan-BET degradation) treatment in MOLT4 and K562 dTAG-BRD4 cells (two biological replicate measurements for each condition).
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| Contributor(s) |
Arnold M, Bressin A, Jasnovidova O, Meierhofer D, Mayer A |
| Citation(s) |
34324863 |
| |
| Submission date |
Oct 02, 2020 |
| Last update date |
Jul 31, 2021 |
| Contact name |
Dr. Andreas Mayer |
| Organization name |
Max-Plack-Institute for molecular genetics
|
| Street address |
Ihnestraße 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (3) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE158966 |
A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3'-processing and termination |
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| Relations |
| BioProject |
PRJNA667099 |
| SRA |
SRP286218 |
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