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| Status |
Public on Dec 21, 2021 |
| Title |
Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To understand the regulatiory mechanisms of trophoblast stemness and differentiation gene expression.
Methods: Three trophoblast stem cell lines were derived from individual term placnta, TSTerm #1 and TSTerm #2 TSTerm #3. The three TSTerm cell lines were differentiated into syncytiotrophoblasts (STB：ST-TSTerm #1, ST-TSTerm #2, and ST-TSTerm #3) and extravillous trophoblasts (EVT：EVT-TSTerm #1, EVT-TSTerm #2 and EVT-TSTerm #3). TS#1GCM1-KO cell line was derived from TSTerm #1 cells by depletion of GCM1 gene. ST-TS#1GCM1-KO cells were differentiated from TS#1GCM1-KO cells. Additionally, CTBTerm #1, CTBTerm #2, and CTBTerm #3 were isolated from individual placentae. The mRNA profiles of tatal fourteen samples as mentioned above were generated by sequencing, using NovaSeq Sequencing System. The raw data were analyzed by CLC Genomics Workbench v10.
Conclusion: We elucidated reciprocal regulation of trophoblast stemness and differentiation gene expression, which facilitated the derivation of TS cells from term placentas.
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| Overall design |
RNA-seq of fourteen cell lines
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| Contributor(s) |
Wang L, Lee Y, Chen H |
| Citation(s) |
35338152 |
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| Submission date |
Oct 01, 2020 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Yun-Shien Lee |
| E-mail(s) |
bojack@mail.mcu.edu.tw
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| Organization name |
Ming Chuan Univ
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| Department |
Dept. Biotechnology
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| Lab |
GMRCL
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| Street address |
TauYau
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| City |
TauYau |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA666919 |
| SRA |
SRP286050 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE158901_FPKM.txt.gz |
3.4 Mb |
(ftp)(http) |
TXT |
| GSE158901_RAWcount.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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