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| Status |
Public on Nov 10, 2020 |
| Title |
Single-cell lineage mapping of a diverse virus-specific naïve CD4 T cell repertoire |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The naïve polyclonal CD4 T cell repertoire can differentiate into multiple lineages during acute viral infection, with some T cell clones exhibiting a preferred lineage choice. TCR α CDR3 motifs can partially influence a clone-intrinsic lineage preference towards TFH fate.
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| |
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| Overall design |
Activated antigen specific CD4 T cells (CD4+CD44+GP66+) were sorted from five C57BL/6J mice and used to perform Single cell gene expression (GEX) and single cell TCR sequencing (TCR) using 10x genomics kit at day 10 post acute LCMV infection
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| |
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| Contributor(s) |
Cui W, Khatun A |
| Citation(s) |
33201171, 36255051 |
| |
| Submission date |
Oct 01, 2020 |
| Last update date |
Nov 16, 2022 |
| Contact name |
Weiguo Cui |
| E-mail(s) |
wcui04@gmail.com
|
| Phone |
312-503-0332
|
| Organization name |
Northwestern University
|
| Department |
Pathology
|
| Lab |
Cui
|
| Street address |
303 E Chicago Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60654 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA666915 |
| SRA |
SRP286042 |
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