|
| Status |
Public on Apr 07, 2021 |
| Title |
Inhibition of the mRNA-binding protein IGF2BP1 suppresses proliferation and sensitizes neuroblastoma cells to chemotherapeutics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gain at chromosome 17q21 in neuroblastoma and is associated with a poor prognosis, independent of MYCN amplification status. Several potential proto-oncogenes have been identified in this region, one of them - insulin-like growth-factor-2 mRNA binding protein (IGF2BP1) – is expressed at high levels in stage 4 tumors, and associated with overall lower patient survival. Here, we demonstrate that down-regulation of IGF2BP1 activity, either by transcript silencing or chemical inhibition, suppresses neuroblastoma cell growth. Furthermore, the combination of IGF2BP1 inhibition along with commonly used chemotherapeutics that broadly affect DNA synthesis, or cyclin-dependent kinase (CDK) inhibitors that disrupt signal transduction, have a synergistic effect on the suppression of neuroblastoma cell proliferation.
|
| |
|
| Overall design |
Total 6 samples were analyzed; 3 replicates each for SK-N-AS RNA shScramble control and SK-N-AS RNA shIGF2BP1.
|
| |
|
| Contributor(s) |
Biegel JM, Dhamdhere M, Gao S, Gowda CP, Imamura Kawasawa Y, Spiegelman VS |
| Citation(s) |
33796454 |
| |
| Submission date |
Sep 20, 2020 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Yuka Imamura Kawasawa |
| E-mail(s) |
yui102@psu.edu
|
| Organization name |
Penn State University
|
| Department |
College of Medicine, Pharmacology
|
| Street address |
500 University Dr.
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
| GSM4795952 |
SK-N-AS RNA shScramble control replicate A |
| GSM4795953 |
SK-N-AS RNA shScramble control replicate B |
| GSM4795954 |
SK-N-AS RNA shScramble control replicate C |
|
| Relations |
| BioProject |
PRJNA664601 |
| SRA |
SRP284043 |
Less...
More...