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Status |
Public on Jul 05, 2022 |
Title |
Single-cell multi-omics in human clonal hematopoiesis reveals that DNMT3A R882 mutations perturb early progenitor states through selective hypomethylation. |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
Clonal hematopoiesis (CH) reflects clonal expansion of blood stem and progenitor cells with somatic mutations. We leveraged multi-modality single-cell sequencing to capture mutation status together with the transcriptome and methylome of CD34+ hematopoietic progenitor cells from five individuals with DNMT3A R882-mutated CH.
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Overall design |
We profiled CD34+ enriched cells from GCSF mobilized bone marrow samples (n = 4) using single-cell RNA sequencing (10X) with targeted genotyping, and single-cell DNA methylation (RRBS) with single-cell RNA sequencing (Smart-Seq2) and targeted genotyping. A 5th CH bone marrow aspirate sample was obtained to validate observed results.
**Submitter declares that the raw data are being deposited in EGA due to patient privacy concerns**
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Contributor(s) |
Dusaj N, Nam A, Izzo F, Murali R, Chaligne R, Ghobrial I, Landau DA |
Citation(s) |
36138229 |
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Submission date |
Sep 16, 2020 |
Last update date |
Nov 29, 2022 |
Contact name |
Neville Dusaj |
E-mail(s) |
ned2010@med.cornell.edu
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Organization name |
Weill Cornell Medicine
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Lab |
Landau Lab
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Street address |
413 E 69th St.
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
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Platforms (3) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (26)
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Relations |
BioProject |
PRJNA663876 |
SRA |
SRP282606 |
SRA |
SRP376370 |