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Series GSE158042 Query DataSets for GSE158042
Status Public on Jan 07, 2022
Title Humans PBMCs: Balanced constitution vs. Phlegm-dampness constitution
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We investigated the expression profiles of lncRNAs and mRNAs in peripheral blood mononuclear cells (PBMCs) from subjects with 13 Phlegm-dampness constitution (PDC) and nine balanced Balanced constitution (BC). The differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) were identified by the “limma” method. Goal was to determine whether long non-coding RNAs (lncRNAs) play a regulatory role in metabolic disease in subjects with PDC.
 
Overall design Two-condition experiment, BC vs. PDC. Peripheral blood mononuclear cells (PBMCs). Biological replicates:9 subjects with Balanced constitution (BC), 13 subjects with Phlegm-dampness constitution samples (PDC). This experiment was performed technically as a dual channel. Cy3 is used to the label of the PDC and the BC. Besides, RNA from all samples was as reference_RNA. And Cy5 is used to label the reference_RNA in each chip. Normalized log2 ratio (Cy3/Cy5) representing test/reference.
 
Contributor(s) Dong L, Li L
Citation(s) 34926685
Submission date Sep 16, 2020
Last update date Jan 08, 2022
Contact name Lidan Dong
E-mail(s) 2018011004@bucm.edu.cn
Phone 13167022696
Organization name 北京中医药大学
Lab National Institute of TCM Constitution and Preventive Medicine
Street address 北京北三环东路11号
City 北京
State/province 北京
ZIP/Postal code 100000
Country China
 
Platforms (1)
GPL20115 Agilent-067406 Human CBC lncRNA + mRNA microarray V4.0 (Probe name version)
Samples (22)
GSM4801971 Balanced constitution_PBMCs Replicate 1
GSM4801972 Balanced constitution_PBMCs Replicate 2
GSM4801973 Balanced constitution_PBMCs Replicate 3
Relations
BioProject PRJNA663843

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158042_RAW.tar 376.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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