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| Status |
Public on Oct 22, 2020 |
| Title |
Dissecting the effect of 3D micro-scaffolds on the transcriptome of Neural Precursors Stem Cells with computational approaches: a focus on mechanotrasduction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
3D cell cultures are becoming more and more important in the field of regenerative medicine due to their ability to mimic the cellular physiological microenvironment. Among the different types of 3D scaffolds, we focus on the Nichoid, a miniaturized scaffold with a structure inspired by the natural staminal niche. The Nichoid can activate cellular responses simply by subjecting the cells to mechanical stimuli. This kind of influence results in different cellular morphology and organization, but the molecular bases of these changes remain largely unknown. Through RNA-seq approach on murine Neural Precursors Stem Cells expanded inside the Nichoid, we investigated the deregulated genes and pathways showing that the Nichoid causes alteration in genes strongly connected to mechanobiological functions. Moreover, we fully dissected this mechanism highlighting how the changes start at a membrane level, with subsequent alterations in the cytoskeleton, signaling pathways, and metabolism, all leading to a final alteration in gene expression. The results shown here demonstrate that the Nichoid influences the biological and genetic response of stem cells thorough specific alterations of cellular signaling. The characterization of these pathways elucidates the role of mechanical manipulation on stem cells, with possible implications in regenerative medicine applications.
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| Overall design |
3 NPCs grown inside the Nichoid (NIC) and replated in standard floating condition for 7 more days
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| Contributor(s) |
Rey F, Pandini C, Barzaghini B, Messa L, Giallongo T, Pansarasa O, Gagliardi S, Brilli M, Zuccotti GV, Cereda C, Raimondi MT, Carelli S |
| Citation(s) |
32942778 |
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| Submission date |
Sep 14, 2020 |
| Last update date |
Oct 22, 2020 |
| Contact name |
Stella Gagliardi |
| E-mail(s) |
stella.gagliardi@mondino.it
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| Organization name |
IRCCS Mondino Foundation
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| Department |
GPG center
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| Street address |
Via Mondino, 2
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| City |
Pavia |
| State/province |
Pavia |
| ZIP/Postal code |
27100 |
| Country |
Italy |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA663306 |
| SRA |
SRP282327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE157897_RAW.tar |
4.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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