|
| Status |
Public on Sep 09, 2020 |
| Title |
Integration of Metabolomics and Transcriptomics Reveals Changes in MRSA Exposed to Terpinen-4-ol |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Staphylococcus aureus (S. aureus) is a gram-positive bacterium that causes a wide range of diseases. Terpinen-4-ol is a monoterpene component contained in most plant essential oils with good antibacterial activity. In this study, terpinen-4-ol effectively inhibited 13 strains of S. aureus, and effectively inhibited the biofilm of MRSA. Metabolomics and transcriptomics were used to elucidate changes in MRSA cells exposed to terpinen-4-ol. Terpinen-4-ol significantly changed (greater than a 2- or less than a 2-fold change) the expression of 304 genes and the level of 847 metabolites (greater than a 1.5- or less than a 0.67-fold change) in MRSA. The levels of genes and metabolites related to the valine, leucine and isoleucine biosynthesis metabolism pathway, the purine and pyrimidine metabolism pathway, energy metabolism and β-lactam resistance were dramatically changed in biofilms exposed to terpinen-4-ol. To the best of our knowledge, this research is the first to report the metabolite and expression profiles of MRSA exposed to terpinen-4-ol.
|
| |
|
| Overall design |
Use 0.08% terpinen-4-ol to treat MRSA ATCC43300
|
| |
|
| Contributor(s) |
Feng C, Yu L, Yanan M, Jianping L |
| Citation(s) |
34736405 |
| |
| Submission date |
Sep 08, 2020 |
| Last update date |
Nov 09, 2021 |
| Contact name |
feng cheng |
| E-mail(s) |
cf1403@sina.com
|
| Organization name |
Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences
|
| Street address |
335 Qilihe Rd,
|
| City |
lanzhou |
| State/province |
GanSu |
| ZIP/Postal code |
730050 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17452 |
Illumina HiSeq 2000 (Staphylococcus aureus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA662311 |
| SRA |
SRP281208 |
Less...
More...