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Series GSE157441 Query DataSets for GSE157441
Status Public on Apr 26, 2021
Title Targeting IRF7 asthma phenotypes through immuno-modulation therapy
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Viral-induced severe asthma exacerbations in children are characterized by IRF7hi and IRF7lo molecular phenotypes. We have developed an experimental animal model that mirrors these response patterns in asthma-resistant PVG and asthma-susceptible BN rats respectively. We aimed to i) characterize the immunological and molecular hallmarks of PVG and BN responses to virus/allergen exposure, and (ii) evaluate the utility of innate immune training with the bacterial lysate OM85 to attenuate ensuing inflammation. Animals were sensitized to OVA/alum, inoculated with murine-adapted Rhinovirus model (vMC0), and challenged with OVA 24h later. RNA-seq was performed on lung and bone marrow at several time points post virus/allergen exposure.
 
Overall design 191 samples in total: 95 lung samples (48 from BN, 47 from PVG) and 96 bone marrow samples (47 from BN, 49 from PVG). Aprox half of samples are from untreated animals, the other half from OM-85 treated animals. Within each strain, tissue and treatment group, samples are further divided into four experimental groups (with n=5-7); group 1 = no OVA or virus, group 2 = no OVA with virus 1 day post infection (DPI), group 3 = OVA and virus 2 DPI, group 4 = OVA and virus 9 DPI)
 
Contributor(s) Lauzon-Joset J, de Jong E, Bosco A
Citation(s) 34367159
Submission date Sep 03, 2020
Last update date Aug 11, 2021
Contact name Emma de Jong
E-mail(s) emma.dejong@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 15 Hospital Ave
City Nedlands, Perth
State/province WA
ZIP/Postal code 6009
Country Australia
 
Platforms (1)
GPL25947 Illumina NovaSeq 6000 (Rattus norvegicus)
Samples (191)
GSM4765053 BN_Untreated_Control_Saline_0_lung_rep1
GSM4765054 BN_Untreated_Virus_Saline_1_lung_rep1
GSM4765055 BN_Untreated_Virus_OVA_2_lung_rep1
Relations
BioProject PRJNA661312
SRA SRP280004

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157441_genecounts_bonemarrow.csv.gz 3.7 Mb (ftp)(http) CSV
GSE157441_genecounts_lung.csv.gz 3.9 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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