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| Status |
Public on Aug 25, 2020 |
| Title |
Gene expression of mouse lung CD11b+ dendritic cells before and after ex vivo culture |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Conventional dendritic cells (cDCs) in the lung drive effector T cell differentiation, and initiate allergic responses upon inhalation of allergens. However, CD11b+ cDCs are composed Ly-6C+ and Ly-6C– subpopulations, and the specific function of each subpopulation has been elusive. Since Ly-6C+ CD11b+ cDCs lost the surface display of Ly-6C upon ex vivo culture, we hypothesized that Ly-6C+ cDCs are precursors of Ly-6C– cDCs. To determine whether 2 CD11b+ cDC subpopulations become the same cell types after maturation, we compared their transcriptomes after ex vivo cultue. As controls, we also compared transcriptomes of freshly isolated Ly-6C+ and Ly-6C– cDCs. Their transcriptomes were also compared with another CD103+ cDCs and monocytes.
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| Overall design |
C57BL/6 mice were inhaled house dust extract together with ovalbumin. Ly-6C+CD11b+, Ly-6C– CD11b+, and CD103+ cDCs and monocytes were purified by flow cytometric sorting. Purified lung cDCs and monocytes were cultured for 2 days. mRNA expression isolated from freshly isolated or cultured cellswas examined by the NanoString platform.
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| Contributor(s) |
Nakano H, Izumi G, Cook DN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 24, 2020 |
| Last update date |
Aug 29, 2020 |
| Contact name |
Rick David Fannin |
| E-mail(s) |
fannin@niehs.nih.gov
|
| Phone |
9842874069
|
| Organization name |
Mr.
|
| Department |
NIEHS/NIH
|
| Lab |
Genomics Core
|
| Street address |
111 Tw Alexander Dr, MD D204
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL25266 |
NanoString Mouse nCounter Myeloid Innate Immunity Panel NS_Mm_Myeloid_V2.0 |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA658991 |
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